Words similar to dsrnas

Example sentences for: dsrnas

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The dsRNAs were introduced in cells essentially as described [ 2 ] . Briefly, A549 cells were propagated in standard MEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin.
Lastly, elevated phosphorylation of eIF-2α has been used as a diagnostic marker of IFN response [ 30 ] . We, therefore, examined the phosphorylation status of eIF-2α in A549 cells following transfection with these dsRNAs.
The anti-F dsRNA also had no effect on P protein levels, suggesting that such dsRNAs do not activate a general antiviral response that might abrogate all viral mRNA translation.
Anti-RSV dsRNAs do not activate an interferon response
To explore these issues, we used dsRNAi to reduce the levels of Arr and DFz2 RNAs in S2 cells in which Wg signaling was stimulated, since others have shown that the expression of endogenous genes in these cells can be eliminated or reduced by direct application of T7-transcribed dsRNAs [ 15 16 ] . Using this approach (Figure 2), we found that the previously observed, approximately 30-fold stimulation of luciferase activity by the Wg expression plasmid was reduced by 70% or more when S2 cells were co-transfected with inhibitory RNAs for Arr, DFz2, or both (Figure 2A), implying that endogenous levels of both types of receptor are required for an efficient response to Wg.