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Example sentences for: dsrnas

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Together, these results directly demonstrate that the dsRNAs promote ablation of the specific mRNA target, which most likely underlies the loss of the respective proteins.
Interestingly, the IFN response requires long dsRNA [ 5 6 ] , and it has been conjectured that the improved specificity of the 21-nucleotide long dsRNA in cultured mammalian cells is probably due to their inability to activate the IFN response [ 2 ] . We provide several lines of direct and indirect evidence that the dsRNAs described here did not activate a general IFN response.
Transfection with dsRNAs was carried out with OligofectAMINE Reagent (Life Technologies) in OPTIMEM I (Life Technologies) as described by the manufacturer for adherent cell lines.
Lastly, elevated phosphorylation of eIF-2α has been used as a diagnostic marker of IFN response [ 30 ] . We, therefore, examined the phosphorylation status of eIF-2α in A549 cells following transfection with these dsRNAs.
The anti-F dsRNA also had no effect on P protein levels, suggesting that such dsRNAs do not activate a general antiviral response that might abrogate all viral mRNA translation.