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Example sentences for: dsrnas

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The dsRNAs were introduced in cells essentially as described [ 2 ] . Briefly, A549 cells were propagated in standard MEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin.
The anti-F dsRNA also had no effect on P protein levels, suggesting that such dsRNAs do not activate a general antiviral response that might abrogate all viral mRNA translation.
Second, growth of VSV is known to be highly sensitive to IFN, however, its replication in A549 cells was not affected by any of the dsRNAs described here (data not shown).
We also provide the first direct evidence that the 21-nt long dsRNAs do not activate a general interferon response.
Transfection with dsRNAs was carried out with OligofectAMINE Reagent (Life Technologies) in OPTIMEM I (Life Technologies) as described by the manufacturer for adherent cell lines.