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Example sentences for: dsrnas

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Transfection with dsRNAs was carried out with OligofectAMINE Reagent (Life Technologies) in OPTIMEM I (Life Technologies) as described by the manufacturer for adherent cell lines.
Anti-RSV dsRNAs do not activate an interferon response
Interestingly, the IFN response requires long dsRNA [ 5 6 ] , and it has been conjectured that the improved specificity of the 21-nucleotide long dsRNA in cultured mammalian cells is probably due to their inability to activate the IFN response [ 2 ] . We provide several lines of direct and indirect evidence that the dsRNAs described here did not activate a general IFN response.
Lastly, elevated phosphorylation of eIF-2α has been used as a diagnostic marker of IFN response [ 30 ] . We, therefore, examined the phosphorylation status of eIF-2α in A549 cells following transfection with these dsRNAs.
When applied against mRNAs of cytoplasmic RNA viruses, the dsRNAs caused degradation of the specific viral mRNA and resultant ablation of the specific viral protein.