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Example sentences for: dsrnas

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The dsRNAs were introduced in cells essentially as described [ 2 ] . Briefly, A549 cells were propagated in standard MEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin.
By 2001, plant scientists working on RNA-directed DNA methylation and TGS had demonstrated a requirement for dsRNAs that are processed to short RNAs, reinforcing a mechanistic link to PTGS (Mette et al.
Transfection with dsRNAs was carried out with OligofectAMINE Reagent (Life Technologies) in OPTIMEM I (Life Technologies) as described by the manufacturer for adherent cell lines.
The extraordinary selectivity of RNAi, combined with its potency—in theory, only a few dsRNAs are needed per cell—quickly made it the tool of choice for functional genomics (determining what a gene product does and with what other products it interacts) and for drug target discovery and validation.
Interestingly, the IFN response requires long dsRNA [ 5 6 ] , and it has been conjectured that the improved specificity of the 21-nucleotide long dsRNA in cultured mammalian cells is probably due to their inability to activate the IFN response [ 2 ] . We provide several lines of direct and indirect evidence that the dsRNAs described here did not activate a general IFN response.