Words similar to digestions
Example sentences for: digestions
How can you use “digestions” in a sentence? Here are some example sentences to help you improve your vocabulary:
Genomic DNA and total RNA were extracted from fibroblast cultures established from skin biopsies as described [ 14 ] . Long-range RT-PCR amplification of FBN1 cDNA fragments and restriction enzyme digestions were performed as previously reported [ 8 ] . Fragments migrating abnormally in agarose gel electrophoresis were excised, purified with the QIAquick Gel Extraction Kit (QIAGEN) and directly sequenced with fluorescent terminators on an ABI Prism 377 DNA sequencer (Perkin-Elmer).
Clustered integrations will, however, be more difficult to analyze in cases where a large number of insertions are present in the particular transgenic line and digestions with multiple restriction enzymes will be necessary to ascertain the structure of the insertion loci.
Current methods to detect tumor clonality include methylation-sensitive restriction enzyme digestions and detection of X chromosome polymorphisms, neither of which give an overall genomic assessment of tumors [ 2].
Digestion yields three fragments (86, 46, and 37 base pairs) in the normal allele and two fragments (123 and 46 base pairs) in the mutant allele as the point mutation at position 1691 is associated with loss of the recognition site for Mnl I. Control digestions were performed with fragments amplified from cloned DNA with and without the factor V Leiden mutation.
Our results from digestions with three different restriction enzymes suggest that there are three insertions in the EL702C line and a single insertion in the EL702E line per haploid genome, consistent with the data obtained with the lac operator probe (Figure 2).
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