Example sentences for: digested

How can you use “digested” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All the plasmid DNAs from these clones were isolated and completely digested by Hind III into linear fragments for run-off transcription.

  • Pst I-digested genomic DNA was electrophoresed through a 1.0% agarose gel, denatured, neutralized, and transferred by capillary blotting to a Hybond -N +membrane (Amersham, Arlington Heights, IL).

  • α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.

  • We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.

  • The pPAI-1-AI was digested with EcoRI and BglII to release a 1426 basepair (bp) PAI-1 coding sequence, which was isolated, purified, and ligated into EcoRI and BglII digested pVL1393.


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