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Example sentences for: digested

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2 μg) were mixed with 0.5 μg of Not I-digested pFA6a-kanMX4 and amplification synthesis was primed with the LI and L4 oligonucleotides.
To enrich for clones with increased survival in human macrophages, the library was passed through THP-1 macrophages as shown schematically in Figure 1. THP-1 is a human monocyte-derived macrophage cell line [ 21 ] and wild-type M. smegmatis bacteria are rapidly killed by THP-1 macrophages [ 22 ] . After three rounds of enrichment, the resulting clones were analyzed individually by Southern blot to evaluate the number of different clones present as previously described [ 20 ] . Out of the 3000 colonies recovered, genomic DNAs from the bacteria of 90 randomly chosen colonies were digested with Pst I and hybridized with the pYUB178 vector.
To obtain the full-length MmCOP1 cDNA, an EST clone (GenBank accession number AI509164) containing the N-terminal region of MmCOP1 cDNA in pT7T3D-Pac vector was obtained from ResGen and digested with XhoI and Hind III.
The radiolabeled PCR products were digested with Alu I or Hpa II, denatured and electrophoresed for 10-16 h at 500 V on non-denaturing 6% acrylamide gels at room temperature [ 24 ] . For RFLP analysis, PCR products were digested with Mbo II and analyzed by electrophoresis on non-denaturing 6% acrylamide gels at 500 V for 3-4 hours.
Cells were digested with mixture of 0.025% trypsin (Gibco BRL) and 0.01% EDTA (Sigma).