Words similar to digested
Example sentences for: digested
How can you use “digested” in a sentence? Here are some example sentences to help you improve your vocabulary:
To make pJC16, we digested pJC10 with Bsp EI and Eco RI to remove a 365 bp fragment.
This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.
BAC DNA samples were digested with Not I to liberate tomato genomic insert DNA then digested with Hind III and separated by electrophoresis in 1% agarose gels.
The PCR product was digested with SpeI (New England Biolabs, Beverly, MA; 20 units) and EcoRI (50 units) in buffer (50 mM NaCl, 100 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 , 0.025% Triton X-100, 100 μg/ml BSA) at 37°C for 60 minutes, purified, and cloned into SpeI/EcoRI digested pTZtd1304.
For a brief summary of these routines and the reasons why the intercepts were not properly digested, see Graham Allison and Philip Zelikow, Essence of Decision, 2d ed.
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