Example sentences for: digested

How can you use “digested” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).

  • The 12 clones encoding unique α s - GFP fusion proteins were digested with Srf I and re-ligated to create full-length fusion proteins (figure 2B).

  • To address these questions, we used an analytical method developed in our laboratory and described in a previous study [ 24 ] . Briefly, the complex cRNA is digested with P1 nuclease and calf intestinal phosphatase and applied to a high performance liquid chromatography (HPLC) column to separate the nucleosides, followed by an absorbance measurement at 260 nm.

  • The PCR amplified product was digested with HindIII and XhoI and subcloned into the correspondingly digested EE-α i2 -pcDNA3.

  • Briefly, DNA equivalent to one fly was restriction digested with Taq I. The DNA was extracted with phenol/chloroform, precipitated with ethanol, resuspended and treated with T4 ligase overnight at 16°C.


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