Example sentences for: digesting

How can you use “digesting” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The bpA sequence along with the 3' AscI site was excised from pbpAAsc by digesting it with SacI and KpnI, and inserted downstream of the MCS in pSAleotpA by digesting it with SacI and KpnI.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.

  • Various deletion constructs were created by first digesting the 3 kb genomic fragment (HindIII-NcoI) with various restriction enzymes, blunted with Klenow DNA polymerase, and then cloned into pGL2Basic luciferase reporter plasmid.

  • These nucleases probably contribute to the repair process, in which SSAPs are involved, by providing the initial break in the dsDNA and/or in digesting the nicked target to generate ssDNA.

  • pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.


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