Example sentences for: digestion

How can you use “digestion” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.

  • Before the reverse transcription reaction, genomic DNA contamination was eliminated by digestion with deoxyribonuclease I (amplification grade; Sigma).

  • Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II Rnase H -Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers.

  • After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.

  • DNA was extracted from tail clips by proteinase K digestion followed by ethanol precipitation.


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