Words similar to digestion
Example sentences for: digestion
How can you use “digestion” in a sentence? Here are some example sentences to help you improve your vocabulary:
Clones were analyzed by restriction digestion, and nucleotide sequencing was carried out on both strands by the dideoxy chain termination method.
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
After further digestion with EcoR I, the NFAT DNA fragment was isolated and ligated into the Sma I and EcoR I sites of pVL1393HAX.
Cells were dissociated in 50 ml tubes by enzymatic digestion with 0.25% Collagenase (Worthington Biochemical, Collagenase Type 2 - CLS 2) and 0.25% Trypsin/EDTA (Invitrogen/Gibco BRL) for 1 hour at 37°C, or until the soft tissues were floating in the solution.
The digestion products were treated with T4 DNA ligase and transformed into E. coli XL 1-Blue.