Example sentences for: digestion

How can you use “digestion” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The control experiment ( Xba I digestion before the run) was done in a CHEF-DR III system (Bio-Rad) under the following conditions: electrophoresis buffer, 0.5 × TBE; 6 V/cm; angle, 120°; pulse time, 4 × 50 sec; run time, 20 hrs; temperature, 14°C.

  • Clonality was further confirmed and X chromosome inactivation status was determined by the length of the polymorphic CAG trinucleotide repeat on the inactive androgen receptor allele [ 21 22 ] . Restriction digestion of four restriction sites between the flanking primers and adjacent to the trinucleotide repeat were carried out with the methylation sensitive enzymes HpaII and HhaI.

  • We also utilized frozen blocks and protein extracts from human placental chorionic villi, and protein extracts from trophoblast cultures and cultured amniotic fibroblasts and placental mononuclear cells [ 46 ] . Placental mononuclear cells were collected from the first digestion, which contains no trophoblast cells [ 46 ] . The study was approved by the Institutional Animal Care and Use Committee and Institutional Review Board.

  • To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.

  • To create p(NFAT) 3 -MLP-G-less a DNA fragment containing the three NFAT sites was recovered from the digestion of the p(NFAT) 3 -E1b-CAT with Xba I and ligated into the Xba I of pΔML200 (gift of J. Parvin) [ 32, 33].

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