Example sentences for: digestion

How can you use “digestion” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DNase I digestion reaction and footprinting gel followed published techniques [ 16 ] .

  • The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.

  • We also utilized frozen blocks and protein extracts from human placental chorionic villi, and protein extracts from trophoblast cultures and cultured amniotic fibroblasts and placental mononuclear cells [ 46 ] . Placental mononuclear cells were collected from the first digestion, which contains no trophoblast cells [ 46 ] . The study was approved by the Institutional Animal Care and Use Committee and Institutional Review Board.

  • Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II Rnase H -Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers.

  • To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.


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