Words similar to digests
Example sentences for: digests
How can you use “digests” in a sentence? Here are some example sentences to help you improve your vocabulary:
The quality of the WGS assembly suggests a strategy for bringing these sequences to high quality: first, select a tiling path of 10-kb genomic clones from the WGS that span each scaffold; second, sequence each clone to high quality; third, assemble these 10-kb sequences to reconstruct the genomic sequence; and fourth, verify the assembly by comparison to cDNA sequence alignments and to restriction digests of genomic DNA, assayed if necessary on Southern blots.
This conclusion is supported by additional Southern analyses of Sca I digests and Eco RI/ Pst I digests of genomic DNA from the parental diploid, the heterozygotes, and the 54 Arg +Ura +segregants (data not shown).
Comparison of the products of these two assemblers with low-resolution restriction enzyme fingerprints of BAC clones has convinced us that constrained assemblies carried out on small genome intervals and more exact comparison to restriction digests are necessary to be truly confident of the fidelity of the sequence assembly, especially in repetitive regions.
We assumed the direct labeling dataset to most accurately represent the true distribution of species in the Alu I and Rsa I digests, since no amplification was required to obtain these data.
We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
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