Words similar to digests
Example sentences for: digests
How can you use “digests” in a sentence? Here are some example sentences to help you improve your vocabulary:
The copy numbers of insertions in these lines were estimated with restriction digests of genomic DNA using Bgl II and Hind III in addition to Pst I, followed by Southern blot analysis using the LacI/NLS fragment as a probe (Figures 1, 2).
We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
To distinguish fragments derived from restriction digests within the cDNA inserts from fragments derived from restriction digests within the vector resulting in mixed cDNA/vector or vector only sequences, the DADA cloning vector contains an engineered segment that is free from the recognition sites used in the fragment analysis (Fig.
PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications.
We assumed the direct labeling dataset to most accurately represent the true distribution of species in the Alu I and Rsa I digests, since no amplification was required to obtain these data.
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