Example sentences for: digests

How can you use “digests” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The copy numbers of insertions in these lines were estimated with restriction digests of genomic DNA using Bgl II and Hind III in addition to Pst I, followed by Southern blot analysis using the LacI/NLS fragment as a probe (Figures 1, 2).

  • PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications.

  • It was subsequently confirmed to be the correct targeting vector by diagnostic PCR, restriction digests and sequencing (data not shown).

  • A set of Bam HI, Eco RI and Hind III digests was used to verify the assembly of 618 BACs.

  • The quality of the WGS assembly suggests a strategy for bringing these sequences to high quality: first, select a tiling path of 10-kb genomic clones from the WGS that span each scaffold; second, sequence each clone to high quality; third, assemble these 10-kb sequences to reconstruct the genomic sequence; and fourth, verify the assembly by comparison to cDNA sequence alignments and to restriction digests of genomic DNA, assayed if necessary on Southern blots.


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