Words similar to denaturing

Example sentences for: denaturing

How can you use “denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

Resultant cDNA was amplified by PCR for 35 cycles using an annealing temperature of 55°C for 30 sec and denaturing temperature of 95°C for 30 sec; the Mg ++concentration in these reactions was 1.5 mM.
To further investigate whether COP1 itself undergoes ubiquitination, a His-tagged ubiquitin expression construct (His-Ub) was co-transfected into the 293 cells with FLAG-COP1, followed by His purification under denaturing condition (in 8 M Urea).
Analysis of the DNAs on denaturing gels revealed that the negative polarity DNA synthesized by the D715V mutant was annealed to a complementary strand [ 23], indicating that it was in an RNA:DNA heteroduplex.
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
5), revealed only one major band corresponding to the spliced and religated A-subunit upon separation in an SDS denaturing gel.