Words similar to denaturing
Example sentences for: denaturing
How can you use “denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:
The resin was washed six times in a denaturing wash buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 6.3) and then boiled for 10 minutes in equal volumes of 2 × SDS loading buffer.
All samples were boiled in Laemmli denaturing buffer and analyzed by Western blotting.
All twelve exons of LMNA were screened by denaturing high performance liquid chromatography (DHPLC) and single strand conformation polymorphism (SSCP) (Figure 4).
Sections of 10 μm thickness were rehydrated and incubated for 10 minutes with one part 30% H 2 O 2 : 9 parts MeOH and processed in HIER (Biotek Solutions), denaturing and blocking solutions following the manufacturer's protocol.
Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).
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