Example sentences for: denaturing

How can you use “denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All twelve exons of LMNA were screened by denaturing high performance liquid chromatography (DHPLC) and single strand conformation polymorphism (SSCP) (Figure 4).

  • Amplifications were performed in a thermal cycler with an initial denaturing step of 5 minutes at 96°C, followed by 25 cycles, with each cycle consisting of 1 minute at 96°C, 50 seconds at 60°C, 50 seconds at 70°C, and a final step of 3 minutes at 72°C.

  • For northern blotting, 20 μg of total RNA was electrophorized on a denaturing agarose gel, transferred to nylon membrane, and hybridized to 33P labeled cDNA probes as described previously [ 11 ] .

  • 2 μm, Schleicher and Schuell) in triplicate in denaturing solution (66 % formamide, 7.9 % formaldehyde, 26 mM MOPS buffer, pH 7.0, 1.3 mM EDTA, 0.066 M sodium acetate) using a slot blot apparatus.

  • The PCR products were separated on a 6% denaturing polyacrylamide gels for 3 hours at 250 voltages and visualized by silver staining.


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