Example sentences for: denaturing

How can you use “denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resin was washed six times in a denaturing wash buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 6.3) and then boiled for 10 minutes in equal volumes of 2 × SDS loading buffer.

  • All samples were boiled in Laemmli denaturing buffer and analyzed by Western blotting.

  • All twelve exons of LMNA were screened by denaturing high performance liquid chromatography (DHPLC) and single strand conformation polymorphism (SSCP) (Figure 4).

  • Sections of 10 μm thickness were rehydrated and incubated for 10 minutes with one part 30% H 2 O 2 : 9 parts MeOH and processed in HIER (Biotek Solutions), denaturing and blocking solutions following the manufacturer's protocol.

  • Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).


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