Example sentences for: denaturing

How can you use “denaturing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR was "hot start", i.e., the polymerase mixed in 10% of the final reaction volume in 1 × buffer was added to the rest of the mix during the initial denaturing step when the sample temperature was already at 94°C.

  • The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.

  • 75 μg total cellular protein was separated by SDS-PAGE on 10% resolving gels under denaturing and reducing conditions.

  • Cells were lysed in a denaturing lysis buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 8.0) and then briefly sonicated in full speed.

  • Sections of 10 μm thickness were rehydrated and incubated for 10 minutes with one part 30% H 2 O 2 : 9 parts MeOH and processed in HIER (Biotek Solutions), denaturing and blocking solutions following the manufacturer's protocol.


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