Words similar to denature
Example sentences for: denature
How can you use “denature” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR primers used for GAPDH and calbindin D were as previously described [ 22 ] . PCR conditions used were: denature at 94°C for 40 s, annealing at 55°C for 50 s with an extension at 72°C for 50 s.
The slides were boiled in deionized water for 3 minutes to denature the printed DNA, dried using low speed centrifugation in a specialized microscope slide-accommodating rotor (Telechem).
4 ± 8.7 U/ug for Arg356Ala and 6.4 ± 2.3 U/μg for Arg356Glu after denature and refolding.
Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.
We conducted the PCR reaction for 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C), followed by a final extension of 4 min at 72°C.