Example sentences for: denature

How can you use “denature” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The slides were boiled in deionized water for 3 minutes to denature the printed DNA, dried using low speed centrifugation in a specialized microscope slide-accommodating rotor (Telechem).

  • Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.

  • Samples were incubated at 94°C for 2 minutes to denature, ramped down at -1 C°/sec to 35°C, held at 35°C for 2 minutes to anneal, ramped down at -0.

  • The PCR primers used for GAPDH and calbindin D were as previously described [ 22 ] . PCR conditions used were: denature at 94°C for 40 s, annealing at 55°C for 50 s with an extension at 72°C for 50 s.

  • 4 ± 8.7 U/ug for Arg356Ala and 6.4 ± 2.3 U/μg for Arg356Glu after denature and refolding.

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