Example sentences for: denature

How can you use “denature” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We conducted the PCR reaction in a MJ Research (Watertown, Massachusetts) PTC-200 Peltier Thermal Cycler programmed with an initial cDNA synthesis and pre-denaturation step of 50°C for 30 minutes and 94°C for 2 minutes followed by then 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C).

  • Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.

  • 4 ± 8.7 U/ug for Arg356Ala and 6.4 ± 2.3 U/μg for Arg356Glu after denature and refolding.

  • Samples were incubated at 94°C for 2 minutes to denature, ramped down at -1 C°/sec to 35°C, held at 35°C for 2 minutes to anneal, ramped down at -0.

  • We conducted the PCR reaction for 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C), followed by a final extension of 4 min at 72°C.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast