Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cycling employed an initial denaturation at 94°C for 4 min, followed by 6 cycles of denaturation at 94°C for 1 min, annealing at 59°C for 15 sec and extension at 72°C for 1 min with a final extension at 72°C for 7 min.

  • The region of interest was amplified by an initial denaturation at 94°C for 5 minutes, 35 cycles of denaturation at 94°C for 30 seconds, annealing at 60°C for 30 seconds, and extension at 72°C for 30 seconds, and concluded with a final extension at 72°C for 10 minutes.

  • The cycling conditions were as follows: initial denaturation at 94°C for 15 min, denaturation at 94°C for 40 sec, annealing at 50-60°C for 40 sec, and extension at 72°C for 90 sec, for 35 cycles, followed by 7 min extension at 72°C.

  • Samples were amplified for 30 cycles in a GeneAmp PCR system 2400 thermocycler (Applied Biosystems) with a three-step cycle of denaturation for 30 s at 94°C, annealing for 30 s at 55°C to 70°C (optimized for each primer pair), and extension for 1 min at 72°C.

  • The reason for none of the clones reacting, could be due to the fact that monoclonal antibodies recognize only an epitope whose tertiary structure might be changed during denaturation conditions of SDS-PAGE.


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