Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The thermal cycling conditions were 40 cycles of the denaturation step at 95°C for 30 seconds, annealing at 60°C for one minute and an extention step at 72°C for one minute.

  • Cycling parameters were as follows: initial denaturation at 94°C for 3 min, 35 cycles of denaturation at 94°C for 30 sec, annealing at 61° for 45 sec, and extension at 72°C for 45 sec, and a final extension at 75°C for 3 min.

  • Immediately following denaturation, the gels were subjected to electrophoresis (42 % urea in 0.5x TBE) to remove the non-acrydited strand.

  • The same volume of denaturation solution (alkaline pH) was added to the tube and incubated for 10 min at room temperature.

  • 5 mM MgCl 2 ). Denaturation and extension conditions were 94°C for 1 min and 72°C for 1 min, respectively.


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