Words similar to denaturation
Example sentences for: denaturation
How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Chromosome and probe denaturation, hybridization, detection, and counterstaining were all done according to standard protocols as recommended by the manufacturer.
(2) Sections were pretreated in PBS containing lysine-rich histone (Sigma) prior to heating and immunostaining; reconstitution with histone restores DNA stability in apoptotic nuclei, thus preventing DNA denaturation and eliminating Mab staining of apoptotic cells.
The region of interest was amplified by an initial denaturation at 94°C for 5 minutes, 35 cycles of denaturation at 94°C for 30 seconds, annealing at 60°C for 30 seconds, and extension at 72°C for 30 seconds, and concluded with a final extension at 72°C for 10 minutes.
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 45 seconds, 63°C for 60 seconds for annealing, and 72°C for 90 seconds for extension.
RNA gels were blotted using the same procedure as DNA gels omitting the depurination, denaturation, and neutralization steps.
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