Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Amplifications were carried out by denaturation for 2 min at 94°C followed by 35 cycles of the following conditions: 94°C for 15 s, 55°C for 45 s, and 68°C for 2 min.

  • This method can produce artifacts, however, because it requires the fixation and permeabilization of cells and denaturation of chromatin before hybridization with labeled single-strand probes [ 12].

  • These reactions were carried out in 50 μ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s; annealing at 65°C for 45 s; and extension at 72°C for 40 s.

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.

  • Apoptotic cells were detected using a monoclonal antibody to single-stranded DNA (ssDNA) (Mab F7-26) according to the method of Apostain, Inc. (Miami, FL) [ 13 ] . Selective binding of anti-ssDNA monoclonal antibody F7-26 to apoptotic nuclei reflects decreased stability of DNA to thermal denaturation.


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