Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR programs for most primer pairs were as follows: 94°C/3 min for initial denaturation; 94°C/30 sec, 65 to 56°C/30 sec, 72°C/1 min, for two cycles at each annealing temperature; 94°C/15 sec, 55°C/30 sec, 72°C/1 min for 20 cycles; and 72°C/10 min final extension [ 23 ] . For some primer pairs, such as PM36, PM 42, PM45, PM50, PM53, and PM60, the PCR conditions should be optimized to find suitable annealing temperature, such as 35 cycles of 94°C/30 sec, 60°C/1 min or 50°C/1 min, 72°C/1 min (see Table 2).

  • For MDR1, GSTπ and TopoIIα genes, after an initial denaturation at 94°C for 1.5 min, 30 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 94°C for 1.5 min, annealing at 54°C for 1 min, and extension at 72°C for 3 min, followed by a final step at 72°C for 10 min.

  • The PCR consisted of 2 pre-incubation steps, 37°C/15 minutes and 95°C/10 minutes (for UNG activation and UNG denaturation/ Taq activation).

  • After denaturation step at 95°C for 5 minutes followed by 30 cycle of denaturation at 92°C (1 min), annealing at 64°C (1 min), extension at 72°C (1.

  • The cycling conditions were as follows: initial denaturation at 94°C for 15 min, denaturation at 94°C for 40 sec, annealing at 50-60°C for 40 sec, and extension at 72°C for 90 sec, for 35 cycles, followed by 7 min extension at 72°C.

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