Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cycling conditions were as follows: denaturation at 95°C/12 min to activate the amplitaq gold, followed by 35 cycles of denaturation at 95°C/45 s, annealing at 60°C/30 s and extension at 72°C/30 s.

  • The cycling conditions were as follows: initial denaturation at 94°C for 15 min, denaturation at 94°C for 40 sec, annealing at 50-60°C for 40 sec, and extension at 72°C for 90 sec, for 35 cycles, followed by 7 min extension at 72°C.

  • Immediately following denaturation, the gels were subjected to electrophoresis (42 % urea in 0.5x TBE) to remove the non-acrydited strand.

  • Thermal cycling parameters included 40 cycles of denaturation at 94°C for 15 seconds, annealing at 40°C for 2 minutes, and extension at 72°C for 30 seconds.

  • Step-down thermo-cycling conditions consisted of 2 min 94°C initial denaturation followed by ten rounds of 94°C for 30 s, 62°C(-1°C/cycle) for 45 s, and 68°C for 3 min.


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