Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For MDR1, GSTπ and TopoIIα genes, after an initial denaturation at 94°C for 1.5 min, 30 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 94°C for 1.5 min, annealing at 54°C for 1 min, and extension at 72°C for 3 min, followed by a final step at 72°C for 10 min.

  • During the early 1990's, alpha-crystallin was shown to act as a molecular chaperone, binding to partially denatured proteins, both in vitro [ 5 ] and probably in vivo [ 6 ] , to inhibit further denaturation and aggregation of lens proteins.

  • Denaturation and electrophoresis of polony gels

  • A typical reaction cycle was denaturation for 3 min at 94°C followed by 35 cycles of denaturation for 15 s at 94°C, annealing for 1 min 15 s at 54°C, and extension for 1 min 15 s at 72°C with a final extension step at 72°C for 10 min.

  • The fact that the tag abundance levels differ slightly more in the libraries made from the same ditag ligation than in those made from the same mRNA sample may reflect a small amount of denaturation of AT-rich ditags during storage of the ligation reaction, as has been previously reported [ 24 ] . Overall, microSAGE passes all the tests for representativity, reproducibility and accuracy used previously for conventional SAGE [ 2 ] .

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