Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This form of denaturation was found to be marginally more effective than placing slides directly onto the heated block for 5 minutes.

  • Step-down thermo-cycling conditions consisted of 2 min 94°C initial denaturation followed by ten rounds of 94°C for 30 s, 62°C(-1°C/cycle) for 45 s, and 68°C for 3 min.

  • PCR cycling conditions consisted of an initial denaturation step at 95°C for 15 min. followed by 35 cycles of 94°C for 5 s, the specified annealing temperature (indicated in Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet) for 15 s, 72°C for 30 s and ending with a final elongation step at 72°C for 3 min.

  • The thermal profile for long-range PCR was as follows: denaturation for 2 min at 94°C, then 35 cycles of denaturation (10 s at 94°C), annealing (30 s at 60°C), and extension (8 min at 68°C), followed by a final extension step of 7 min at 68°C.

  • Cycling parameters were as follows: initial denaturation at 94°C for 3 min, 35 cycles of denaturation at 94°C for 30 sec, annealing at 67°C for 45 sec, and extension at 72°C for 90 sec, and a final extension at 75°C for 5 min.


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