Words similar to denaturation
Example sentences for: denaturation
How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Amplification was performed using an ABI 5700 machine (Applied Biosystems, Foster City, CA, USA) with a hot start at 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min for denaturation, annealing and elongation.
Cycling parameters were an initial denaturation of 94°C for 4 min, followed by 30 cycles of denaturation at 94°C for 1 min, annealing for 45 sec and extension at 72°C for 1 min 30 sec, followed by a final extension of 72°C for 7 min.
After denaturation, the highly repetitive sequences in the probe mixture DNA were allowed to bind to the C o t1 DNA (10 min at 37°C).
Amplifications were carried out by denaturation for 2 min at 94°C followed by 35 cycles of the following conditions: 94°C for 15 s, 55°C for 45 s, and 68°C for 2 min.
The hepadnaviral P protein possesses many unique features relative to the better studied reverse transcriptases of the retroviruses: (i) it uses its own amino-terminal domain as a protein primer for initiation of DNA synthesis [ 7, 9, 10], (ii) it cannot be isolated in an active form from virions without partial proteolysis or denaturation [ 13, 14, 15], and (iii) under normal circumstances it is active only on the endogenous pregenomic RNA that is encapsidated with it in the viral cores [ 16].
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