Words similar to denaturation
Example sentences for: denaturation
How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:
The amplification reaction consisted of the following: 50°C for 2 min, denaturation at 95°C for 10 minutes, and 40 thermal cycles of 95°C for 15 s and 60°C for 1 min.
Apoptotic cells were detected using a monoclonal antibody to single-stranded DNA (ssDNA) (Mab F7-26) according to the method of Apostain, Inc. (Miami, FL) [ 13 ] . Selective binding of anti-ssDNA monoclonal antibody F7-26 to apoptotic nuclei reflects decreased stability of DNA to thermal denaturation.
PCR reactions (10 mM Tris.HCl, pH 8.3, 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each deoxyribonucleoside triphosphate, each primer at 2 μM, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus) involved 35 cycles of denaturation (95°C for 1.0 min), primer annealing (55°C for 1.0 min), and primer extension (72°C for 2 min).
The PCR programs for most primer pairs were as follows: 94°C/3 min for initial denaturation; 94°C/30 sec, 65 to 56°C/30 sec, 72°C/1 min, for two cycles at each annealing temperature; 94°C/15 sec, 55°C/30 sec, 72°C/1 min for 20 cycles; and 72°C/10 min final extension [ 23 ] . For some primer pairs, such as PM36, PM 42, PM45, PM50, PM53, and PM60, the PCR conditions should be optimized to find suitable annealing temperature, such as 35 cycles of 94°C/30 sec, 60°C/1 min or 50°C/1 min, 72°C/1 min (see Table 2).
Step-down thermo-cycling conditions consisted of 2 min 94°C initial denaturation followed by ten rounds of 94°C for 30 s, 62°C(-1°C/cycle) for 45 s, and 68°C for 3 min.