Example sentences for: denaturation

How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The thermal profile for long-range PCR was as follows: denaturation for 2 min at 94°C, then 35 cycles of denaturation (10 s at 94°C), annealing (30 s at 60°C), and extension (8 min at 68°C), followed by a final extension step of 7 min at 68°C.

  • Thermal cycler parameters included 2 min at 50°C, 10 min at 95°C, and 40 cycles involving denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min.

  • The cycling conditions were as follows: denaturation at 95°C/12 min to activate the amplitaq gold, followed by 35 cycles of denaturation at 95°C/45 s, annealing at 60°C/30 s and extension at 72°C/30 s.

  • After synthesis of the first-strand cDNA, PCR was performed by initial denaturing at 94°C for 2 min followed by 28 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 1 min, and extension at 72°C for 1.5 min.

  • Cycling conditions consisted of 35 cycles with denaturation steps at 94°C for 30 s, hybridization steps at 55°C for 30 s and an extension step at 72°C for 1 min.


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