Words similar to denaturation
Example sentences for: denaturation
How can you use “denaturation” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR reactions (10 mM Tris.HCl, pH 8.3, 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each deoxyribonucleoside triphosphate, each primer at 2 μM, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus) involved 35 cycles of denaturation (95°C for 1.0 min), primer annealing (55°C for 1.0 min), and primer extension (72°C for 2 min).
Cycling parameters were an initial denaturation of 94°C for 4 min, followed by 30 cycles of denaturation at 94°C for 1 min, annealing for 45 sec and extension at 72°C for 1 min 30 sec, followed by a final extension of 72°C for 7 min.
The resulting products were amplified using nested PCR with the Advantage-GC 2 PCR kit (CLONTECH, Cat# K-1913-Y) and two 30 cycle rounds with each cycle having two steps (denaturation 94°C, 30 sec.
The rat Abcg5 -specific primers (RG5c-F1 and RG5Fc-R1), Abcg8 -specific primers (RG8c-F1 and RG8c-R1) and β-actin primers (sense: 5'-CTACGTCGCCCTGGACTTCGAGC-3', antisense: 5'-GATGGAGCCGCCGATCCACACGG-3') were used for further PCR amplification (an initial denaturation at 94°C for 3 min, 35 cycles of PCR amplification with each cycle consisting of 30 min at 94°C, 45 s at 60°C, and 2 min at 72°C, followed by an extension step of 72°C for 5 min).
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 45 seconds, 63°C for 60 seconds for annealing, and 72°C for 90 seconds for extension.
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