Words similar to denaturation

Example sentences for: denaturation

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The process of formamide denaturation, hybridization, and extension was repeated 3 additional times in order to perform an extension with each of the four dNTPs and completely sequence each position.
The PCR programs for most primer pairs were as follows: 94°C/3 min for initial denaturation; 94°C/30 sec, 65 to 56°C/30 sec, 72°C/1 min, for two cycles at each annealing temperature; 94°C/15 sec, 55°C/30 sec, 72°C/1 min for 20 cycles; and 72°C/10 min final extension [ 23 ] . For some primer pairs, such as PM36, PM 42, PM45, PM50, PM53, and PM60, the PCR conditions should be optimized to find suitable annealing temperature, such as 35 cycles of 94°C/30 sec, 60°C/1 min or 50°C/1 min, 72°C/1 min (see Table 2).
The PCR cycling conditions consisted of a denaturation step at 94°C for 30 s, an annealing step at 54°C (S12) or 60°C (TNF-α), an extension step at 72°C for 1 min, and a final extension step at 72°C for 10 min.
First cycle: 5 min denaturation at 95°C, 3 min annealing at 58°C and 90 second extension at 72°C; cycles 2-35, 80 second denaturation, 30 second annealing, and 90 second extension, with a final extension of 5 min.
After denaturation, the highly repetitive sequences in the probe mixture DNA were allowed to bind to the C o t1 DNA (10 min at 37°C).