Example sentences for: denatured

How can you use “denatured” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sections were prehybridized for 2 h at 54°C in 500 μl hybridization buffer (50% formamide, 5x SSC, 40 μg/ml salmon sperm DNA) before hybridization with heat denatured RNA probes which had been diluted to 400 ng/ml in hybridization buffer.

  • The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.

  • A rabbit genomic library in Lamda DASH ®II vector (Stratagene) was screened with [ 32P]-labeled full-length human EP 2 (gift from Dr. D. Woodward, Allergan Pharmaceuticals) [ 20 ] . The filters were fixed by ultraviolet light exposure (Stratalinker, Stratagene) and hybridized 18 hours at 56°C in a solution containing 6X SSC, 5X Denhardt's, 0.1% SDS, 150 μg/ml denatured herring sperm DNA and 3 × 10 6cpm/ml labeled probe.

  • RNA samples were analyzed by denatured gel electrophoresis.

  • Fifteen μl each of the adapterized HL60 cDNA and HL60/TLK286 cDNA were mixed with 30 μl of 2x HB buffer in a final volume of 60 μl, denatured at 95°C for 5 min and annealed at 68°C for 20 hours.


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