Example sentences for: denatured

How can you use “denatured” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2μg of RNA was denatured with 0.5μM of random decamer primers in a total volume of 12μl, and heated to 77°C for three minutes and placed on ice.

  • Hybridization, wash and detection conditions were then applied as above except that chromosomes were denatured by placing slides directly onto a heating block set at 75°C for 7 min.

  • The denatured Cy3 and Cy5 DNA were mixed and loaded onto the area of the twelve selected spots on the microarray chip.

  • The resulting 35 μl probe solution was denatured at 95°C for 2 min and then incubated at 42°C for 25 min.

  • The labeled DNA was mixed with 50 μl of 100 mM sodium acetate (pH 5.2), purified with Qiagen PCR Purification Kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.


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