Example sentences for: denatured

How can you use “denatured” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • RNA was denatured with glyoxal, fractionated via agarose gel electrophoresis, and immobilized on a positively charged nylon membrane.

  • Hybridization was performed overnight at 67°C in a sealed bag with 125 μl/sq cm fresh hybridization solution containing formamide and salmon sperm DNA as above with the addition of 40 ng/ml digoxigenin labeled probe denatured by boiling for 5 minutes.

  • Fibrosarcoma PAI-1, wtPAI-1 or its mutants were denatured with 6 M guanidine-hydrochloride (Gdn-HCl) (45 min at 37°C), followed by dialysis (4-16 hours at 4°C) against 50 mM sodium acetate, pH 5.6, containing 0.5 M NaCl and 0.01% Tween-20.

  • These variations were not seen when samples were denatured in SDS immediately following extract preparation (data not shown), suggesting that they arise from varying degrees of deneddylation presumably occuring during the immunoprecipitation step.

  • Initially, 2, 4, or 6 ug of RNA was combined with 2 ug of oligo dT (ResGen) and denatured at 70°C for 10 minutes.


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