Example sentences for: denatured

How can you use “denatured” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Interestingly, the specific inhibitory activity of wtPAI was 63 ± 15 U/ug just after purification, and was increased to 84 ± 21 U/ug after denatured by 6 M Gdn-HCl and refolded in 50 mM sodium acetate (pH 5.6) at 4°C.

  • The hybridization probes (see below) were denatured for 5 min at 80°C and added to the hybridization mix (400 ng/ml).

  • Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).

  • single strand) probe fragments was checked by agarose gel electrophoresis of denatured probe followed by Southern transfer and demonstration of biotin by BluGene kit (Invitrogen Life Technologies).

  • Nothing like him in Bosch or Breugel, nothing so denatured as to resemblenot a semblance of a human facebut the substance of some form made and then unmade, or like a lump of human butter excavated from a bog.


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