Example sentences for: pbs

How can you use “pbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Tissue sections were fixed with 4% paraformaldehyde in 1x PBS (or alternatively methanol) for 30 min and blocked for 60 min in 2% BSA in 1x PBS.

  • Aliquots of 300 μl (approximately 150,000 cells) were placed in 5-ml Falcon tubes and washed in PBS.

  • After another wash with 1 ml 0.5% Tween-20/1% BSA/PBS, nuclei were resuspended in 0.25 ml PBS containing 5 μg/ml Propidium Iodide, filtered through 1.2 ml Costar cluster tubes (Costar #4410), or 6 ml tubes with 35 μm strainer cap (Falcon 2235, Becton-Dickinson) in case of volumes greater than 0.5 ml.

  • Rat neonatal microglia on glass coverslips were fixed and permeabilized in -20°C methanol for 10 min, and then blocked with 10% goat serum in phosphate buffered saline (PBS) at 25°C for 5 min.

  • Sections incubated without the primary antibody but with PBS were used as negative controls.

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