Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).

  • Next we created a human myocilin expression construct where the N-terminal 14aa were deleted (pcDNA3.hMYOC.ΔN14) forcing translation to begin at the second methionine codon (Fig.

  • EE-tagged α i2 (1-331)-pcDNA3 was constructed by by PCR amplification of the coding region for the amino acids 1 to 331 of α i2 using the T7 primer as the 5' primer and 5'-ccggctcgagtcacttggtgtcggtggcgcatg-3' as the 3' primer and using EE-α i2 -pcDNA3 as the template.

  • This fragment was excised with Not I and subcloned into the Not I site of the mammalian expression vector pcDNA3 (Invitrogen), in either the sense or antisense orientation.

  • The genes were amplified by PCR and cloned into pcDNA3 enabling in vitro transcription.


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