Example sentences for: pbs-tween

How can you use “pbs-tween” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).

  • The pegs were then immersed for 2 h in a trough containing 14 ml of anti-human IgG F(ab'2) fragment conjugated to alkaline phosphatase in PBS-Tween and developed with nitrophenyl phosphate (Sigma Chemical Co.

  • After washing 3x with PBS-Tween (DPTC-treated PBS for the mRNA), plates are probed with an anti-M13 antibody conjugated to horseradish peroxidase (1:3000 in PBS-NFM) for 1 hour at room temperature followed by addition of 100 μl of ABTS for 15-30 minutes at room temperature.

  • After overnight incubation, excess IgG antibody from the serum was washed away by thrice transferring the pegs to troughs containing 14 ml of PBS containing 0.05 % Tween 20 (PBS-Tween) for 5 min each time.

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