Example sentences for: pbs-t

How can you use “pbs-t” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Proteins resolved by SDS-PAGE were transferred to nitrocellulose membranes and the membranes blocked for 2 hours in 5% dry milk in PBS-T (Phosphate Buffered saline and 0.1% Tween 20).

  • The blot was washed once for 5 minutes in PBS-T, incubated with goat-anti-rabbit conjugated HRP (1:10000 dilution) for 1 hour, washed for 5 minutes in PBS-T and developed with Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc.

  • The primary antibody was monoclonal mouse anti-hemagglutinin (dilution 1:1000 in PBS-T, gift of Guojon Bu, Washington University, St. Louis), and the secondary antisera was horseradish peroxidase-conjugated sheep anti-mouse IgG (dilution 1:1000 in PBS-T, Amersham, Arlington, IL).

  • These samples were separated by SDS-PAGE, electrophoretically transferred to polyvinylidene difluoride membrane, and blocked with 5% milk in PBS-T.

  • 1% v/v Tween 20 (PBS-T) overnight at 4°C, then subjected to immunoblotting and ECL (Amersham, Arlington, IL) detection.


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