Example sentences for: pbs-ks

How can you use “pbs-ks” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).

  • pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.

  • The PCR product was digested with Xba I and Mro I and ligated into pBS-KS +-NFATp that had been digested with Xba I and Mro I. pVL1392-HAX-NFATp(1-722) was made by subcloning a Nde I - Msc I fragment from pBS-KS +-NFATp(NdeI) into the Nde I and Sma I sites of pVL1392-HAX.

  • First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.

  • pVL1393-HAX-NFATp(331-921) was made by digesting pBS-KS +-NFATp with SgrA I and filling in the overhang by treatment with Klenow.


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