Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).

  • To inactivate the γ 1 gene, a 4.4 kb Bam HI/ Xho I fragment upstream of exon 1 was subcloned into a multiple cloning site 5' of the neo and Herpes virus TK gene that was in pBluescript (Stratagene).

  • The resulting PCR product was cloned into the Eco RV site of pBluescript KS+ and clones were screened for the correct orientation.

  • The specific probes were prepared by digesting the appropriate pBluescript plasmid with XbaI and EcoRI , separating the fragment on a 1.5% agarose gel followed by extraction using the Qiaex II extraction kit (Qiagen).

  • The vector for all constructs was pBluescript(-) (Stratagene).


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