Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • RACE products were then subcloned into pbluescript KS II +using TA vector cloning.

  • Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T

  • The resulting PCR product was cloned into the Eco RV site of pBluescript KS+ and clones were screened for the correct orientation.

  • Each PCR product was gel-purified and cloned into the Eco RV site of pBluescript KS+ (Stratagene).

  • Hybridization probes were derived from a ~600-bp cDNA that contains the entire coding region of the human VEGF 165 [ 8 ] subcloned into the pBluescript II KS+ vector (Stratagene, La Jolla, CA).

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