Words similar to pbluescript
Example sentences for: pbluescript
How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).
To inactivate the γ 1 gene, a 4.4 kb Bam HI/ Xho I fragment upstream of exon 1 was subcloned into a multiple cloning site 5' of the neo and Herpes virus TK gene that was in pBluescript (Stratagene).
The resulting PCR product was cloned into the Eco RV site of pBluescript KS+ and clones were screened for the correct orientation.
The specific probes were prepared by digesting the appropriate pBluescript plasmid with XbaI and EcoRI , separating the fragment on a 1.5% agarose gel followed by extraction using the Qiaex II extraction kit (Qiagen).
The vector for all constructs was pBluescript(-) (Stratagene).