Example sentences for: tris-buffered

How can you use “tris-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • TBS: Tris-buffered saline.

  • Adherent and non-adherent cells were dissolved in Tris-buffered saline (pH 7.6) containing 10 mM EDTA and 0.5% sarkosyl, and 1 mg/ml proteinase K. After digestion (~16 h) of cellular proteins at 55°C, DNA was sequentially extracted with phenol/chloroform and chloroform.

  • Briefly, membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST) and non-specific binding sites were blocked by immersing the membrane in blocking reagent (0.

  • Membranes were blocked in 5% nonfat dried milk for one hour, washed in 10 mM Tris-buffered saline (pH 7.5) containing 0.1% Tween-20 (TBST) and probed with primary antibody overnight at 4°C.

  • Alkaline phosphatase activity was measured as follows: Cells were rinsed in cold TBS (Tris-buffered Saline), scrapped from the plate, homogenized in 0.05% Triton X-100 in Tris-HCl (pH 7.5) on ice, and the sample was centrifuged at 1200 rpm for 5 minutes at 4°C.


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