Words similar to tris-buffered

Example sentences for: tris-buffered

How can you use “tris-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

After washing in Tris-buffered saline with tween 20 (TBST), blots were incubated for one hour at room temperature with HRP-conjugated secondary antibody, washed again and detected by chemiluminescence.
The membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST), blocked by immersing the membrane in 0.5% casein in TBST for 1 h then incubated at 4°C overnight with affinity purified goat anti-ERβ N-19 antibody (Santa Cruz Biotechnology) diluted 1:4000 in blocking reagent.
Nitrocellulose membranes were blocked with 5% nonfat milk in Tris-buffered saline Tween (20 mM Tris, 137 mM NaCl, pH7.
Blots were incubated overnight with anti-Tie1 and anti-Tie2 antibodies (R&D Systems) at 1:5000, in Tris-buffered saline Tween containing 5% nonfat milk.
Adherent and non-adherent cells were dissolved in Tris-buffered saline (pH 7.6) containing 10 mM EDTA and 0.5% sarkosyl, and 1 mg/ml proteinase K. After digestion (~16 h) of cellular proteins at 55°C, DNA was sequentially extracted with phenol/chloroform and chloroform.