Words similar to tris-buffered

Example sentences for: tris-buffered

How can you use “tris-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

Adherent and non-adherent cells were dissolved in Tris-buffered saline (pH 7.6) containing 10 mM EDTA and 0.5% sarkosyl, and 1 mg/ml proteinase K. After digestion (~16 h) of cellular proteins at 55°C, DNA was sequentially extracted with phenol/chloroform and chloroform.
After 2 hours of incubation at 37°C, cells were washed one time with PBS and subsequently lysed in 0.5 ml of lysis/wash buffer containing 1% NP40 in Tris-buffered saline plus an EDTA-free protease inhibitor cocktail (Roche; Indianapolis, USA) for 20 minutes at 4°C.
Blots were incubated overnight with anti-Tie1 and anti-Tie2 antibodies (R&D Systems) at 1:5000, in Tris-buffered saline Tween containing 5% nonfat milk.
The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.
After repeated washing with Tris-buffered saline (TBS, 0.9% NaCl, 10 mM Tris, pH7.