Example sentences for: tris-buffered

How can you use “tris-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • TBS: Tris-buffered saline.

  • Membranes were blocked in 5% nonfat dried milk for one hour, washed in 10 mM Tris-buffered saline (pH 7.5) containing 0.1% Tween-20 (TBST) and probed with primary antibody overnight at 4°C.

  • Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.

  • Nitrocellulose membranes were blocked with 5% nonfat milk in Tris-buffered saline Tween (20 mM Tris, 137 mM NaCl, pH7.

  • Blots were incubated overnight with anti-Tie1 and anti-Tie2 antibodies (R&D Systems) at 1:5000, in Tris-buffered saline Tween containing 5% nonfat milk.


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