Words similar to tris-buffered

Example sentences for: tris-buffered

How can you use “tris-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

The blots were preincubated for 30 minutes at 22°C in Tris-buffered saline containing 5% nonfat powdered milk and 0.2% Tween-20 (TBS-T), and were then probed with affinity purified anti-AQP1 IgG (1:5000) for 2 hr at 22°C.
TBS: Tris-buffered saline.
Briefly, two identical membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST) and non-specific binding sites were blocked by immersing the membrane in blocking reagent (0.
Nitrocellulose membranes were blocked with 5% nonfat milk in Tris-buffered saline Tween (20 mM Tris, 137 mM NaCl, pH7.
After washing in Tris-buffered saline with tween 20 (TBST), blots were incubated for one hour at room temperature with HRP-conjugated secondary antibody, washed again and detected by chemiluminescence.