Words similar to trishcl

Example sentences for: trishcl

How can you use “trishcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

PCR was performed in 50 μL reactions containing: template, 50 pmol each of primers F3 (5'-CATAACGCCAGCCCACCTACTG-3') and ETn1 (5'-CACGATTTGTGGGTAAAATAGGAG C-3'), 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.
For each gene, 5 μl of cDNA was incorporated into a 25 μl PCR mixture containing 8.8 mM TrisHCL, 44 mM KCl, 1.3 mM MgCl 2 , 0.00088 % gelatin, 0.284 mM each deoxynucleoside triphosphate, 1 U of Taq DNA polymerase (Applied Biosystems, Foster City, CA) and 1.78 μM of each primer.
The cell pellet was resuspended in 0.5 ml spheroplast buffer [1 M sucrose, 25 mM TrisHCl (pH 8.0), 5 mM EDTA, and lysozyme (50 μg ml -1)].
Standard RT-PCR reactions were performed with each 50 μL reaction containing 1/8 of an RT reaction, 50 pmol each of forward and reverse primer, 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.