Words similar to trishcl

Example sentences for: trishcl

How can you use “trishcl” in a sentence? Here are some example sentences to help you improve your vocabulary:

Standard RT-PCR reactions were performed with each 50 μL reaction containing 1/8 of an RT reaction, 50 pmol each of forward and reverse primer, 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.
PCR was performed in 50 μL reactions containing: template, 50 pmol each of primers F3 (5'-CATAACGCCAGCCCACCTACTG-3') and ETn1 (5'-CACGATTTGTGGGTAAAATAGGAG C-3'), 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.
The cell pellet was resuspended in 0.5 ml spheroplast buffer [1 M sucrose, 25 mM TrisHCl (pH 8.0), 5 mM EDTA, and lysozyme (50 μg ml -1)].
For each gene, 5 μl of cDNA was incorporated into a 25 μl PCR mixture containing 8.8 mM TrisHCL, 44 mM KCl, 1.3 mM MgCl 2 , 0.00088 % gelatin, 0.284 mM each deoxynucleoside triphosphate, 1 U of Taq DNA polymerase (Applied Biosystems, Foster City, CA) and 1.78 μM of each primer.