Words similar to tris-acetate

Example sentences for: tris-acetate

How can you use “tris-acetate” in a sentence? Here are some example sentences to help you improve your vocabulary:

5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
Each of the four transcripts, dap, lys, phe and thr , were pooled, and fragmented in fragmentation buffer (40 mM Tris-acetate pH8.
500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.
cRNA (10 μg) was fragmented at 94°C for 35 min in buffer containing 40 mM Tris-acetate pH 8.1, 100mM KOAc, 30 mM MgOAc.
cRNA yield was quantitated by measuring the UV absorbance at 260 nm, and fragmented in 40 mM Tris-acetate (TrisOAc), pH 7.9, 100 mM KOAc, and 31.