Words similar to tris-acetate

Example sentences for: tris-acetate

How can you use “tris-acetate” in a sentence? Here are some example sentences to help you improve your vocabulary:

500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.
Protein samples were subjected to electrophoresis on 3-8% gradient polyacrylamide gels (Novex) in Tris-acetate buffer containing 0.1% Na dodecyl SO 4 , according to the manufacturer's directions.
Each of the four transcripts, dap, lys, phe and thr , were pooled, and fragmented in fragmentation buffer (40 mM Tris-acetate pH8.
5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
The IVT product was chemically fragmented with 40 mM Tris-acetate, pH 8.1, 125 mM KOAc and 30 mM MgOAc at 94°C for 35 minutes.