Words similar to tris-acetate
Example sentences for: tris-acetate
How can you use “tris-acetate” in a sentence? Here are some example sentences to help you improve your vocabulary:
500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.
cRNA yield was quantitated by measuring the UV absorbance at 260 nm, and fragmented in 40 mM Tris-acetate (TrisOAc), pH 7.9, 100 mM KOAc, and 31.
15 μg aliquots of each cRNA sample were fragmented at 95°C for 35 min in 40 mM Tris-acetate, pH8.
were incubated in reaction buffer (50 mM Tris-acetate (pH 7.5), 150 mM potassium acetate, 10 mM magnesium acetate and 4 mM spermidine) at 37°C for 2 hr in a 10 μL reaction.
cRNA (10 μg) was fragmented at 94°C for 35 min in buffer containing 40 mM Tris-acetate pH 8.1, 100mM KOAc, 30 mM MgOAc.
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