Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).

  • Oocytes were prepared after the method of Rho et al . [ 29 ] . Typically 30 oocytes were lysed by trituration in 200 μL of a solution containing 100 mM Tris-HCl, 100 mM NaCl, 0.5% Triton X-100, 0.05 mg/mL pepstatin, 0.05 mg/mL leupeptin, and 0.05 mg/mL aprotinin (pH 8.0) and the homogenate was incubated for 15 minutes on ice.

  • , IL) as described previously [ 50 81 ] . To determine the detergent solubility of the Rab24(D123I) mutant, transfected cells from a 10 cm culture dish were homogenized in 0.2 ml of 100 mM Tris-HCl, pH 7.4, containing 0.5% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, and 2 mM EDTA.

  • 5 M Tris-HCl, pH6.

  • 3 nM) was incubated with varying concentrations of the wild type enzyme and its mutant derivatives in a total volume of 10 μL containing 50 mM Tris-HCl, pH 7.8, 5 mM MgCl 2 and 0.01 % BSA.


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