Words similar to tris-
Example sentences for: tris-
How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each amplification reaction contained 50 mM KCl, 10 mM tris-HCl pH 8.4, 1.5 mM MgCl 2 , 0.25 μM of each primer, 200 μM dNTP, 10% DMSO, 0.1 μg DNA and 1 U Tag polymerase in a final volume of 20 μl.
Eluted fractions were evaluated by spectrophotometry and immunoblot analysis [ 42 ] . Fractions with the highest absorbance at 280 nm and the strongest reactivity by immunoblot were neutralized in 1 M Tris-HCl (pH 9.5) buffer and stored at 4°C.
After sonication with a Sonic Dismembrator Model 300 with microtip (Fisher), 100 μl of the mixture was added to 50 μl of 3 × reaction buffer containing 150 mM Tris-HCl, pH 8.0; 30 mM thymidine; 3 mM DTT; 5 mM MgCl 2 , 0.3 mM GTP.
All ligation reactions were in 1x Ampligase Reaction Buffer supplied by Epicentre Technologies: 20 mM Tris-HCl (pH8.
We used a 5'- 32P labeled 30-mer synthetic U5-PBS RNA template annealed with a complementary 30-mer DNA to determine the RNase H activity of the enzymes [ 31 ] . The reaction mixture contained labeled RNA-DNA hybrid (10 K Cerenkov cpm), 50 mM Tris-HCl pH 8.0, 60 mM KCl, 10 mM dithiothreitol, 0.1 mg/ml bovine serum albumin, 5 mM MgCl
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