Words similar to tris-
Example sentences for: tris-
How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:
For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).
For mock permeabilization of cores, the glycine and Tris-HCl used to lower the pH and to neutralize the solution during permeabilization were mixed together prior to addition to core particles.
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.
One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
Proteins were transferred onto nitrocellulose membranes (Genie blotter, Idea Scientific Company, Minneapolis, MN, USA) for 1 hour, at 24 V and 4°C, using transfer buffer composed of 25 mM Tris-HCl, 192 mM glycine and 10% methanol.
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