Words similar to tris-
Example sentences for: tris-
How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:
After incubation, Coplin jars were removed from the water bath and slides immersed in sodium citrate or Tris-HCl and were allowed to sit in solution for a further 20 minutes before being rinsed with water and air-dried.
The membranes were blocked overnight at 4°C in TBST (10 mM Tris-HCl, 150 mM NaCl, 0.05% Tween; pH 8.0) containing 3% fat free dry milk.
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
Excess antibody was removed by two 15 min washes in Buffer 1, and the sections were equilibrated for 5 min in Buffer 2 (Tris-HCl 100 mM, NaCl 100 mM, and MgCl 2 50 mM, pH 9.5).
Each PCR included 2 μl of the RT reaction containing the primary strand cDNA, reaction buffer [10 mM Tris-HCl (pH 8.4), 50 mM KC1, 1.5 mM MgCl 2 , 0.001% gelatin], 2 μM of each dNTP, 0.2 μM of the same anchored primer used in the RT reaction, 0.2 μM of one of the eight 13-mer arbitrary primers API-8 (Table 1), 12.