Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All ligation reactions were in 1x Ampligase Reaction Buffer supplied by Epicentre Technologies: 20 mM Tris-HCl (pH8.

  • The cells were washed once with buffer containing 50 mM Tris-HCl, pH7.

  • The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.

  • Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).

  • Smooth muscle strips were weighed and total protein was extracted by glass-glass homogenization using 50 μl per milligram dry tissue sodium dodecyl sulfate (SDS)-based extraction buffer: 25 mM Tris-HCl, pH 7.4, 2% SDS, 10% glycerol, 1 mM dithiothreitol (DTT), 1 μM leupeptin, 10 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium orthovanadate, 5 mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF).


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