Words similar to tris-

Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

After rinsing, the peroxidase activity was revealed with 3,3'-diaminobenzidine (Sigma, 15 mg/50 ml Tris-HCl in the presence of 0.01% hydrogen peroxide).
Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).
Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.
After 24 h incubation at 37°C, the culture medium was pre-cleared and the virus was concentrated by centrifugation at 13,500 rpm for 80 min in a SW28 rotor and resuspended in 1 mM Tris-HCl (pH 9).
Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.