Words similar to tris-

Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

Equal amounts of denatured, subtracted cDNA from each tester were spotted on four separate, positively charged nylon membranes (Roche Applied Science, Indianapolis, IN) using the BioDot apparatus (BioRad, Hercules, CA) and neutralized with 200 μl neutralization solution (1 M Tris-HCl, pH 8.0, 1.5 M NaCl).
Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.
Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.
The mixture was packed into a 10 ml Polyprep chromatography column (Bio-Rad) and washed with 10-column volumes of buffer P. The bound material was eluted with 10-column volumes of 3 M NaCl, desalted with 20 mM Tris-HCl pH 8.0, 10% glycerol, treated for protein depletion, dialyzed against deionized water, lyophilized and resuspended in deionized water.
The DNA was precipitated, washed in 70% ethanol, and dissolved in buffer containing 10 mM Tris-HCL (pH 7.5) and 1 mM EDTA.