Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nonspecific binding was blocked with 5% fat free milk in TBST [50 mM Tris-HCL (pH7.

  • The cells were collected, resuspended in a buffered solution of 10 mM Tris-HCl (pH 9) and homogenized with 20 strokes in a Dounce homogenizer.

  • The Cy5 and Cy3 labelled probes were combined and purified in a Microcon ®YM-30 column (Millipore) by washing three times with Tris-EDTA buffer.

  • Duplicates of membrane preparations equivalent to 0.1 mg protein were incubated with 125I-EGF (50 or 100 ng; Amersham Life Sciences, Arlington Heights, IL) in 200 μl reaction buffer (5 mM Tris-HCl, pH 7.0, 125 mM sucrose, 75 mM NaCl, 0.5 mM Ca 2+and 0.5% BSA) at 25°C for 60 min.

  • Briefly, genomic DNA (50 ng) was amplified using 50 pmol primers (5'-CAT TCG CAC TCT GGA GTC-3' and 5'-AGG CTC TTG GGG TAC TTG-3') in GeneAmp PCR buffer (50 mM KCl, 10 mM Tris-HCl, pH 8.3, 0.001% [w/v] gelatin, 1.5 mM MgCl


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