Words similar to tris-

Example sentences for: tris-

How can you use “tris-” in a sentence? Here are some example sentences to help you improve your vocabulary:

For protein depletion, CM was mixed with subtilisin at 0.4 μg/ml in 20 mM Tris-HCl pH 8.0, 10% glycerol and incubated at 37°C for 16 h.
The following buffer solutions were tested: 2X SSC (300 mM NaCl, 30 mM sodium citrate), 2X SSPE (300 mM NaCl, 20 mM NaH 2 PO 4 , 2 mM EDTA), and, 2X STE (300 mM NaCl, 50 mM Tris-HCl, 2 mM EDTA).
Equal amounts of protein lysates from each condition in 1X sample buffer (125 mM Tris-HCl, pH 6.8, 1% β-mercaptoethanol, 2% SDS, 5% glycerol, 0.003% bromphenol blue) were separated on 7.5 or 10% polyacrylamide gels and subsequently transferred to nitrocellulose membranes.
To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).
Recombinant HA-tagged NFATp proteins (approximately 500 ng) were incubated with calcineurin (500 ng, Sigma) and calmodulin (200 ng, Sigma) in 21 μl of buffer that had the following final concentrations of components: 7 mM Tris-HCl, 5 mM Hepes (pH 7.6), 90 mM NaCl, 15 mM betamercaptoethanol, 0.3 mM DTT, 1 mM EDTA, 30% (v/v) glycerol, 3 mM MgCl 2 , and 1.5 mM MnCl 2 . Control reactions were performed with NFATp proteins in the absence of added calcineurin and calmodulin.