Words similar to tris

Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

After 48 hours cells were harvested by scraping in PBS, centrifuged at 1,000 × g for 10 minutes, and gently resuspended in around 10 ml of a 50 mM Tris pH 7.5, 250 mM sucrose buffer containing an EDTA-free protease inhibitor cocktail (Roche).
Protein lysates from whole cells and tissues were prepared with a buffer, which contained 50 mM Tris, pH 7.5, 150 mM NaCl, 1% (v/v) Nonidet P40 (NP40), 2 mM 4-(2-aminoethyl)benzenesulphonyl fluoride, 1 μg/ml leupeptin, 2 μg/ml aprotinin and 2 μg/ml pepstatin.
Alternatively, the bound material was first eluted with 2 column volumes of 8 M urea buffer (8 M urea, 0.1 M NaH 2 PO 4 , 10 mM Tris, pH 8.0), followed by washing with 10 column volumes of NP40 Lysis buffer, and then removing any remaining bound material by boiling the Sepharose beads in SDS sample buffer.
Following centrifugation at 700 ×g, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.
7, as compared to Tris buffer at pH 7.1, can be ascribed mainly to the much higher pH values and ionic strength of the phosphate systems.