Words similar to tris
Example sentences for: tris
How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:
After the Agrobacterium culture (2 ml) was collected by centrifugation in a microfuge, the pellet was resuspended in a solution of 50 mM glucose, 25 mM Tris and 10 mM ethylene aminotetraacetic acid (pH8.
Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).
Staining was stopped by a 10 min wash in Tris/EDTA (10/1 mM, ph 8.0), and non-specific staining was removed by 1 hour in 95% EtOH.
Serial dilutions of the proteases were made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl 2 . Enzyme activity was measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA (Bachem Bioscience, Torrance, CA), 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer.
The recombinant protein was purified on anti-HA-conjugated beads and washed two times in TGEMD buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 5 mM MgCl 2 , 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT) containing 1 M NaCl and an additional two times in TGEMD buffer containing 0.2 M NaCl.
Loading...