Words similar to tris

Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

Blots were blocked at room temperature for 1 hour in TBS-T (10 mM Tris, pH 8.0, 150 mM NaCl, 0.1% Tween-20) containing 5% dried fat-free milk, then incubated overnight at 4°C in TBS-T containing 0.5% milk and primary Ab (0.
For SDS/PAGE experiments on disulfide bonds, the oocytes were treated as above, except that the lysis buffer contained either 100 mM DTT or 20 mM Tris (2-Carboxyethyl) Phosphine Hydrochloride (TCEP) (Pierce, Rockford, IL).
Cell lysates for immunoprecipitation were prepared by disrupting cells in immunoprecipitation buffer (20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF).
For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg cdk2 antibody in 300 μl IP buffer [50 mM Tris (pH 7.5), 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, and 1% NP-40] and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.