Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cell membranes were pelleted by centrifugation at 26,000 × g and then resuspended in 10 mM Tris buffer.

  • Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.

  • Cells were incubated at 37°C in lysozyme solution (5 mg/ml lysozyme, 50 mM Tris pH 7.5, 10 mM EDTA pH 8.0), followed by addition of 20% SDS containing 0.3% beta-mercaptoethanol.

  • After addition of 2.5 μl 1 N Tris/HCl (pH 7.5), labeled cDNA was concentrated by ETOH precipitation and resuspended in 2 μl water.

  • The cells were lysed with 1% NP-40 buffer (150 mM NaCl, 50 mM Tris, pH 8.0) containing 100 μg/mL phenylmethylsulfonyl fluoride and 1 μg/mL aprotinin, sonicated for 30 seconds, then frozen.


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