Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.

  • Another significant factor may be the difference in charge between the buffer salts; phosphate is anionic whereas Tris is cationic and zwitterionic.

  • Precipiates were washed four times in 20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMS, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.

  • The reactions were allowed to sit in the dark for 45 min and then quenched by the addition of a tenth volume 1 M pH 8 Tris base (a 500-fold molar excess of quencher).

  • The recombinant protein was purified on anti-HA-conjugated beads and washed two times in TGEMD buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 5 mM MgCl 2 , 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT) containing 1 M NaCl and an additional two times in TGEMD buffer containing 0.2 M NaCl.


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