Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).

  • 125 M Tris, 2% SDS, 10% sucrose, 5% 2-mercaptoethanol, and 0.02% bromophenol blue at a pH of 8.0) was added to each tube and the tubes were heated at 95°C for 5 minutes.

  • BAECs were mechanically harvested, washed, and resuspended in 500 μl lysis buffer (10 mM Tris pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.01% Triton X-100).

  • The resin was washed six times in a denaturing wash buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 6.3) and then boiled for 10 minutes in equal volumes of 2 × SDS loading buffer.

  • Mouse tissues were homogenized in the RIPA lysis buffer (150 mM NaCl, 1.0% NP-40, 0.5% DOC, 0.1% SDS, 50 mM Tris, pH 8.0) at a concentration of 0.2 g tissue per ml of buffer.


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