Example sentences for: tris

How can you use “tris” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Immunoprecipitates were collected by centrifugation (11000 × g for 10 min at 4°C), washed five times with 500 μl TG-buffer containing 20 mM Tris, 250 mM NaCl, and 0.5% NP-40.

  • HeLa nuclear extract (5 ng) in reaction buffer (100 mM Tris.HCl, pH 7.5, 50 mM KCl, 5 mM MgCl 2 , 0.1 mM EDTA, 15 μg/ml BSA) was incubated with 5 μg of purified GST or GST-BTBD2 on ice for 15 min before the addition of 0.25 μg plasmid and incubation at 30°C for 0-24 min.

  • Both the supernatant and the pellet were diluted by adding 6X sample buffer (10% SDS, 1.2 mg/mL bromphenol blue, 0.6 M DTT, 30% glycerol, .1 M Tris/HCl pH 6.8) and heated to 80°C for 15 minutes to denature the protein.

  • Cells were resuspended in lysis buffer (10 mM Tris (pH 7.5), 1 mM EDTA, 50 mM NaCl, 0.25% Nonidet P-40, 1 mM PMSF, 1 mg of aprotinin/mL, 0.1 mM sodium vanadate, 10 mM sodium molybdate, and 10 mM β-glycerol phosphate) and lysed by sonication with a microtip on setting 2 and 20% duty cycle for 10 s.

  • Sections were etched in 0.1 N HCl for 5 min., rinsed three times for five min each in TBS (25 mM Tris, 140 mM NaCl, 2.7 mM KCl, pH 8.0), placed in blocking buffer for 30 min (0.


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