Words similar to transfected
Example sentences for: transfected
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6Cshows that the II-III loop antibody recognized the C-terminus half when cells were transfected with fs-α 1S but not when myotubes were transfected with fs-α 1S M701I.
DNA fragments containing AQP1 DNA were liberated from pSKAC after restriction and ligated into adenovirus as described previously [ 27 ] . Human embryonic kidney cells (293 cells) were transfected with ligation mixture and individual viruses were isolated from cell lysates by two consecutive rounds of plaque purification using an agar overlay as described previously [ 27 ] . Individual viruses were amplified in 293 cells and purified over cesium step gradient.
GTM66 cells were derived from a 92-year old woman with glaucoma and were cultured in low glucose DMEM (HyClone Laboratories) with 10% FBS (HyClone Laboratories) and antibiotics (Invitrogen) as described [ 11 17 ] . Cells were transfected with Lipofectamine 2000 reagent according to the manufacturers recommendations (Invitrogen).
The transfected receptors are expressed at four fold the level of the endogenous receptors.
Specificity in Western blotting and flow cytometry was evaluated by detection of exogenous TTP in transfected 293 cells and by blocking this activity with peptide [ 24 ] .