Words similar to transfected

Example sentences for: transfected

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For transfections, 10 5HepG2 cells were plated in 24 well dishes with DMEM supplemented with 10% charcoal stripped serum and transfected using the calcium phosphate precipitation method as described [ 16 ] . The next morning, cells were washed with phosphate buffered saline and ligands were added.
Thus parental and PLCγ2-deficient cells were transfected with kinase active Btk:ER and stable clones selected that expressed equivalent levels of Btk:ER, as measured by anti-Btk Western blotting of whole cell lysates (Fig 4A).
Soluble and particulate fractions were isolated as previously described [ 29 ] . BHK or COS-7 cells were transfected in 6 cm plates with 3 μg of the indicated expression plasmid.
Several heat shock proteins have been found in cell nuclei in the absence of stress [ 30 ] , and alpha-crystallin has been shown to interact directly with DNA [ 18 ] . AlphaB, expressed in transfected CHO cells, has been shown to ectopically localize to interphase nuclei, suggesting a regulatory role for this protein in the nucleus [ 19 ] . A subset of immortalized lens epithelial cells from alphaBKO mice have been shown to hyperproliferate [ 20 ] suggesting that alphaB may be important in maintaining genomic stability.
Figure 4shows a series of representative photographs of transfected 3T3 cells that were co-stained with antibody against the Rab24 myc epitope, combined with DAPI to visualize the chromatin (Fig.