Words similar to transfecting

Example sentences for: transfecting

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HSV-1 strain SC16 (wild type laboratory virus), HSV-2 SB5 (wild type laboratory virus), HSV-2 6652 (wild type clinical virus), HSV-2 83D (wild type clinical virus), HSV-2 6757 (high error mutant clinical virus) were described previously [ 21 ] and the HSV-1 PAA r5 (antimutator laboratory virus) utilized in this study was a kind gift of D. Coen (Harvard University, Boston, MA) [ 10 ] . HP66 (HSV-1 genome containing a LacZ insertion within the pol coding region) viral DNA was kindly provided by D. Coen (Harvard University, Boston, MA) [ 17 ] . HP66 viral stocks were prepared by transfecting HP66 viral DNA into PolB3 cells, which allow for virus propagation by expressing the viral polymerase.
Mouse L cells secreting Wnt-3A were established in our lab, by transfecting expression constructs in which the Wnt-3A cDNA [ 34 ] is driven by the PGK promoter.
Our results confirm the findings of others that show the possibility of transfecting post-mitotic, fully differentiated cells using PEI [ 2, 23].
We first addressed this issue by transiently transfecting a heterologous promoter containing a consensus AP-1 site fused to the luciferase reporter gene [ 11 ] along with wild type RARα or S77A mutant expression vectors.