Words similar to transfecting
Example sentences for: transfecting
How can you use “transfecting” in a sentence? Here are some example sentences to help you improve your vocabulary:
Mouse L cells secreting Wnt-3A were established in our lab, by transfecting expression constructs in which the Wnt-3A cDNA [ 34 ] is driven by the PGK promoter.
HSV-1 strain SC16 (wild type laboratory virus), HSV-2 SB5 (wild type laboratory virus), HSV-2 6652 (wild type clinical virus), HSV-2 83D (wild type clinical virus), HSV-2 6757 (high error mutant clinical virus) were described previously [ 21 ] and the HSV-1 PAA r5 (antimutator laboratory virus) utilized in this study was a kind gift of D. Coen (Harvard University, Boston, MA) [ 10 ] . HP66 (HSV-1 genome containing a LacZ insertion within the pol coding region) viral DNA was kindly provided by D. Coen (Harvard University, Boston, MA) [ 17 ] . HP66 viral stocks were prepared by transfecting HP66 viral DNA into PolB3 cells, which allow for virus propagation by expressing the viral polymerase.
Our results confirm the findings of others that show the possibility of transfecting post-mitotic, fully differentiated cells using PEI [ 2, 23].
We first addressed this issue by transiently transfecting a heterologous promoter containing a consensus AP-1 site fused to the luciferase reporter gene [ 11 ] along with wild type RARα or S77A mutant expression vectors.