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Example sentences for: transfectants

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We then introduced into each line a plasmid containing a luciferase reporter downstream of multimerized Gli binding sites and a minimal promoter [ 20 ] . An isolated stable clone of the 10T1/2 cell transfectants (referred to as clone S12) gave a 10-20-fold up-regulation of luciferase activity (Figure 1a) when stimulated with Hh protein [ 21 ] for 24 hours.
Where indicated, GFP-Hyal-1, GFP-Hyal-2 and GFP stable transfectants were treated with TNF (50 ng/ml) for 0, 20, 40, 60 and 120 minutes.
Wild-type and mutated receptors in the pRc/CMW vector were transfected into CHO-K1 cells using calcium phosphate precipitation technique as described previously [ 21 ] . Stable transfectants were selected with 500 μg/ml G-418 (Geneticin; Life Technologies) and screened by intact cell binding using [ 3H]rauwolscine as previously described [ 21 ] .
For the syk -negative DT40 transfectants the medium was supplemented with 2 mg/ml hygromycin B (Calbiochem, San Diego, CA).
Stable transfectants were selected in 400 mg/ml G418 over a period of 4 weeks and subsequently maintained in the absence of G418 in normal growth medium.