Words similar to transfectants
Example sentences for: transfectants
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We then introduced into each line a plasmid containing a luciferase reporter downstream of multimerized Gli binding sites and a minimal promoter [ 20 ] . An isolated stable clone of the 10T1/2 cell transfectants (referred to as clone S12) gave a 10-20-fold up-regulation of luciferase activity (Figure 1a) when stimulated with Hh protein [ 21 ] for 24 hours.
The ability of the sense and antisense transfectants to deplete tryptophan from culture medium was determined following 48 hours in culture.
For the syk -negative DT40 transfectants the medium was supplemented with 2 mg/ml hygromycin B (Calbiochem, San Diego, CA).
In contrast, antisense transfectants formed aggregates, which exhibited markedly different morphologies and differed from sense-transfected aggregates in two principal respects; shape and size (Fig 5D,E,F).
The Hyal-1 and Hyal-2 stable transfectants were pretreated with TGF-β1 for 1 hr and then treated with TNF overnight.