Words similar to transfect
Example sentences for: transfect
How can you use “transfect” in a sentence? Here are some example sentences to help you improve your vocabulary:
This finding underscores the versatility of PEI as a method for introducing genes into postmitotic populations of cells, particularly as our previous efforts to transfect sympathetic neurons using lipid-based reagents (Lipofectamine and Lipofectin) had been unsuccessful.
GTM66 cells were chosen for this study based on their ability to grow and transfect well and are a glaucomatous TM cell line derived from a 92-year-old female donor [ 11 17 ] . Transfection of GTM66 cells with the N-terminal deletion mutant resulted in a distribution pattern similar to wild-type human myocilin (Fig.
The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.
Plasmid DNA alone is not very efficient transfectant, therefore the electroporation offers a possibility to transfect tumours resistant to viral infection [ 11 12 ] . In this study, the electroporation of a non-permissive adenocarcinoma of the lung is compared to the adenoviral vector.
5 ul) of the reaction mixtures, containing full length viral RNA, were used to transfect approximately 2 × 106 Monkey LLC-MK2 cells in phosphate-buffered saline (PBS) by electroporation in a 0.4 cm gap electroporation cuvette.