Words similar to transfect

Example sentences for: transfect

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Plasmid DNA alone is not very efficient transfectant, therefore the electroporation offers a possibility to transfect tumours resistant to viral infection [ 11 12 ] . In this study, the electroporation of a non-permissive adenocarcinoma of the lung is compared to the adenoviral vector.
have made attempts to improve the electroporation protocol by introducing new settings, consisting in the combination of high voltage and low voltages pulses, that are inducing less permeabilisation of the myocytes [ 22 ] . Even if these combined protocols seems to be slightly less toxic, there is still much improvement needed to compete with the new generations of adenoviral vectors as a method to transfect muscle in patients.
The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.
Equal amount of total plasmids were used to transiently transfect COS-1 using the DEAE dextran method [ 40 ] . A GFP construct was used to monitor the transfection efficiency.
5 ul) of the reaction mixtures, containing full length viral RNA, were used to transfect approximately 2 × 106 Monkey LLC-MK2 cells in phosphate-buffered saline (PBS) by electroporation in a 0.4 cm gap electroporation cuvette.