Words similar to transfect
Example sentences for: transfect
How can you use “transfect” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 ul) of the reaction mixtures, containing full length viral RNA, were used to transfect approximately 2 × 106 Monkey LLC-MK2 cells in phosphate-buffered saline (PBS) by electroporation in a 0.4 cm gap electroporation cuvette.
Plasmid DNA alone is not very efficient transfectant, therefore the electroporation offers a possibility to transfect tumours resistant to viral infection [ 11 12 ] . In this study, the electroporation of a non-permissive adenocarcinoma of the lung is compared to the adenoviral vector.
The human bile acid binder (HBAB) cDNA [ 37 ] was cloned into a derivative of pCep4 (Invitrogen Corp., Carlsbad, CA), that had been previously modified by deleting the EBNA 1 gene [ 38 ] . The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.
Equal amount of total plasmids were used to transiently transfect COS-1 using the DEAE dextran method [ 40 ] . A GFP construct was used to monitor the transfection efficiency.
This finding underscores the versatility of PEI as a method for introducing genes into postmitotic populations of cells, particularly as our previous efforts to transfect sympathetic neurons using lipid-based reagents (Lipofectamine and Lipofectin) had been unsuccessful.