Words similar to transesterification

Example sentences for: transesterification

How can you use “transesterification” in a sentence? Here are some example sentences to help you improve your vocabulary:

E. coli DNA topoisomerase I and the ZD domain were expressed and purified as described [ 6 41 ] . To express the 67 kDa N-terminal transesterification domain (Top67), a stop codon at amino acid 598 was introduced into plasmid pJW312 [ 42 ] used for topoisomerase I expression by site-directed mutagenesis employing the Chameleon-Mutagenesis kit from Stratagene.
The transesterification domains of these eukaryotic enzymes have high degrees of identity to E. coli DNA topoisomerase III [ 7 37 ] . However, the decatenation loop is not present in the eukaryotic topoisomerase III sequences and to date the decatenation activity has not been demonstrated for these enzymes.
The second transesterification results in exon ligation with the release of lariat RNA complexed with the intron-encoded protein.
Using purified 67 kDa transesterification domain and 30 kDa ZD domain, results from experiments described here provide new insight into the action of these two individual domains in the enzyme mechanism.
To gain further details for this model of enzyme action, we are characterizing the protein-protein interactions between the Top67 transesterification domain and the ZD domain, as well as the protein conformational changes that can take place when the enzyme interacts with DNA substrate.