Example sentences for: tpa

How can you use “tpa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To induce macrophage differentiation, 10 nM phorbol ester (TPA) was added to growth cultures at a cell density of 1.5 × 10 6cells/ml, 40 hrs prior to harvest.

  • [ 10 ] demonstrated a retardation of granule cell migration in tissue plasminogen activator (tPA) knock-out mice.

  • To provide the evidence to support the conclusion obtained from enzyme assay, certain amount of wtPAI-1, E351R and E351A were incubated with equal amount of tPA at 37°C for 30 minutes before put on ice, at same time these PAI-1s were incubated in PBS at 37°C for 3 hours, respectively, before the PAI-1s were incubated with same amount of tPA at 37°C for 30 minutes, then the protein samples prepared at two time points were subjected to SDS-PAGE and western blot as described before.

  • In order to prove that p44/42 MAPK can be stimulated in H441 cells by an activator of this kinase, some cells were also treated with 10 nM 12- O -tetradecanoylphorbol-13-acetate (TPA), a strong stimulator of p44/42 MAPK in H441 cells, for 5 min [ 21 ] . TPA caused an approximately twofold increase in p44/42 MAPK phosphorylation over control levels.

  • To elucidate the mechanism through which such mutations affect the activity of PAI-1, we determined their effect on the functional stability of PAI-1 and on its ability to form an SDS stable complex with either tPA or uPA.


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