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Example sentences for: trna
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In particular, a series of nested deletions of the P6 stem-loop have been shown to partially or completely compromise ribozyme activity [ 15 ] . More importantly, either magnesium or the tyrosyl tRNA synthetase from Neurospora mitochondria (CYT18) can suppress many of these defects [ 16 17 ] . Previous studies with other introns have also revealed that deletion of the P5 stem-loop can modulate ribozyme activity [ 18 19 ] . We hypothesized that sites where deletions modulated ribozyme activity might also prove to be sites where aptamer conformational changes would modulate ribozyme activity.
The alignments that were based on another laboratory's initial effort and enlarged and refined for the CRW project are: 1) the prokaryotic (Archaea and Bacteria) alignments for 16S rRNA [ 85 ] ; 2) the 5S rRNA alignments [ 55 ] ; and 3) the tRNA alignments [ 81 ] . The group I and II intron alignments were originally based upon sequences collected by Michel [ 32 34 ] .
For example, the S. cerevisiae phenylalanine tRNA sequence, with a length of 76 nucleotides, has 37 possible helices (as defined above); only four of these are in the crystal structure.
Our interpretation follows Muto and Osawa's seminal demonstration of the high correlation between the GC content of noncoding, exon, intron, tRNA and rRNA sequences, which indicates that a common force influences the composition of all parts of the genome [ 53].
The fact that a rule could be drawn suggests that relatively simple interactions are involved, possibly between the tRNA in the P site, the incomming potential suppressor tRNA and the message, as it has been shown in yeast [ 28].
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