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Example sentences for: trna
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Briefly, covariation analysis, a specific application of comparative analysis (as mentioned earlier), searches for helices and base pairs that are conserved in different sequences that form the same functionally equivalent molecule ( e.g., tRNA sequences).
The purified probe was adjusted to a total volume of 26 μl and mixed with 5.3 μl 20X saline-sodium citrate (SSC), 1 μl yeast tRNA (10 μg/μl), 2 μl poly(A) DNA (10 μg/μl), and 0.6 μl 10% sodium dodecyl sulfate (SDS).
In particular, a series of nested deletions of the P6 stem-loop have been shown to partially or completely compromise ribozyme activity [ 15 ] . More importantly, either magnesium or the tyrosyl tRNA synthetase from Neurospora mitochondria (CYT18) can suppress many of these defects [ 16 17 ] . Previous studies with other introns have also revealed that deletion of the P5 stem-loop can modulate ribozyme activity [ 18 19 ] . We hypothesized that sites where deletions modulated ribozyme activity might also prove to be sites where aptamer conformational changes would modulate ribozyme activity.
Release 1 reported 292 tRNAs [ 3]; two tRNA genes were deleted as a result of sequence finishing resolving repeated regions of the genome.
As expected, this list includes several tRNA synthetases, which were previously found to be frequently transferred [ 6 7 8 ] , and enzymes involved in DNA repair ( cf . [ 9 ] ). More surprisingly, this list also includes translation initiation factors and several ribosomal proteins.