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Example sentences for: trna

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Structural comparisons using the DALI program [ 20], indicated that the core fold shared by the KilA-N and APSES domains is more distantly related to the LAGLIDADG site-specific DNA endonucleases and the amino-terminal domain of the tRNA splicing endonucleases (TEN domain) (Figure 1b) [ 21].
To hybridize the arrays, purified and labeled cDNA targets were dissolved in 100 μl total volume of ExpressHyb solution (Clontech, Palo Alto, CA) containing 8 μg of polydA 40-60 (Amersham Pharmacia), 2 μg of yeast tRNA (Gibco-BRL), 10 μg of human Cot I DNA (Gibco-BRL).
E. coli has 86 tRNAs, and most of these appear to be present in the ancestor (the parsimony criterion for presence of a tRNA in the ancestor, based on distribution in sequenced genomes, is somewhat unreliable because of sequence homology among different tRNA genes).
All of the secondary structure base pairs and a few of the tertiary structure base pairs predicted with covariation analysis [ 5 6 7 8 9 71 72 73 74 ] are present in the tRNA crystal structure [ 10 11 ] . The analysis of fragments of 5S rRNA [ 75 ] and the group I intron [ 76 ] resulted in similar levels of success.
One exception to this is the tRNA analysis, which was initially performed with the Mixy chi-square-based algorithm [ 71 ] , and thus the color codes are based on that analysis.