Words similar to topoisomerase
Example sentences for: topoisomerase
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As a result, Top67 could position itself in the absence of ZD domain at the junction of double- and single-stranded DNA for subsequent DNA cleavage, as previously observed for intact topoisomerase I [ 8 ] . Reversal of DNA cleavage also took place readily with Top67 upon addition of 1 M NaCl and 4 mM MgCl 2 . The ZD domain also was not required for selectivity of a cytosine in the -4 position relative to the cleavage sites.
The requirement of specific protein-protein interactions between the two domains could also account for the weak relaxation activity observed for the hybrid topoisomerase with ZD linked to topoisomerase III sequence [ 21 ] .
Meiotically induced, Spo11 (Rec12)-dependent double-strand DNA (dsDNA) breaks have been demonstrated in budding yeast and fission yeast [ 12 13 14 15 ] . Spo11 almost certainly catalyzes these breaks, as it shares high homology with the A subunit of Topoisomerase VI of Sulfolobus shibatae [ 16 17 ] and it becomes linked by a phosphotyrosine linkage to the 5' end of the cleaved DNA [ 18 19 20 21 ] . Spo11 homologs have been identified in a wide range of eukaryotes, and available evidence suggests that initiation of meiotic recombination via Spo11-dependent dsDNA break formation is conserved [ 22 23 24 25 ] . Our results with the fission yeast S. pombe fully support this view (see results and discussion).
Human DNA topoisomerase I (Topo I) is a 765-amino-acid nuclear enzyme [ 9] involved in topological changes of DNA structure [ 10].
Our studies on nmt55 and those of p54 nrbhave characterized this protein as an RNA binding protein with the ability to associate with Topoisomerase I and the polypyrimidine tract-binding protein associated splicing factor (PSF) [ 16 17 18 19 20 ] . Further, we observed the association of nmt55/p54 nrbwith several splicing factors known to be essential for spliceosome formation suggesting a role for nmt55/p54 nrbin pre-mRNA splicing (unpublished results).
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