Words similar to topoisomerase
Example sentences for: topoisomerase
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The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.
aa, amino acid; BTB: Broad-Complex, Tramtrack and Bric-a-brac; GST, glutathione-S-transferase; NLS, nuclear localization signal; ORF, open reading frame; RT-PCR, reverse transcription - polymerase chain reaction; TOP1, DNA topoisomerase I.
Rec12 (Spo11) protein shares homology with the catalytic (Top6A) subunit of Topoisomerase VI, which functions as a heterotetramer with Top6B [ 16 17 ] . However, no Top6B homolog is present in most eukaryotes and the Rec12 (Spo11) enzyme does not need to affect strand passage in the manner of canonical type II topoisomerases [ 11 ] . We therefore tested whether overexpression of rec12 +would lead to a hyper-recombination phenotype, thereby implicating function as a homodimer.
This result indicated that with regard to the mechanism of E. coli topoisomerase I, the ZD domain was likely to function as a single-stranded DNA binding domain instead of having double-stranded DNA binding function as previously suggested [ 21 ] . Even though Zn(II) binding transcription factors that recognise specific double-stranded DNA are well represented in eukaryotes [ 23 24 ] , there are also numerous examples of Zn(II) coordination being required for interaction with single-stranded nucleic acid or damaged DNA with single-strand characteristics [ 24 25 26 27 ] .
The presence of this decatenation loop instead of the Zn(II) binding ZD domain in topoisomerase III may account for the dominance of the decatenation activity over the relaxation activity.
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