Words similar to topoisomerase
Example sentences for: topoisomerase
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The double-stranded DNA-binding activity in E. coli topoisomerase III required for highly efficient decatenation activity is attributed to a 17-amino-acid residue with no counterpart in E. coli topoisomerase I [ 28 29 ] . It may be required for interaction with the passing double-strand of DNA in the decatenation mechanism.
We used CPT, an inhibitor of topoisomerase I, that is a well defined apoptogenic agent belonging to a promising class of antineoplastic drugs [ 23 ] . In contrast to more physiological stimuli (i.e, oxidative stress) induction of apoptosis by CPT is less complex and thus is a better model for study of MP-release phenomenon.
There are two homologous type IA topoisomerases present in E. coli . Topoisomerase III has potent DNA decatenating activity for resolution of plasmid DNA replication intermediates, but much weaker relaxation activity than topoisomerase I [ 17 ] . To exhibit maximal relaxation activity, topoisomerase III requires high temperature (52°C) along with low magnesium and monovalent ion [ 17 18 ] . In contrast, E. coli topoisomerase I was not active in the in vitro assay for resolution of plasmid DNA replication intermediates [ 19 ] . Removal of the C-terminal 49 amino acids from the 653 amino acid topoisomerase III protein resulted in drastic reduction of catalytic activity [ 20 ] . Fusion of the carboxyl-terminal 312 amino acid residues of E. coli topoisomerase I, which includes the entire ZD domain, onto the 605 N-terminal amino acids of topoisomerase III generated a hybrid topoisomerase that has relaxation activity resembling topoisomerase III along with weak decatenating activity [ 21 ] . Although preferring single-stranded DNA as binding substrate, topoisomerase I had been shown to also bind double-stranded DNA [ 22 ] , but there is no data available to indicate which domain in the enzyme is responsible for this interaction.
ADR = adriamycin; DRO = droloxifen; GSTπ = glutathione S-transferase Pi; MDR = multidrug resistance; MG = megestrol; MRP = MDR-related protein; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H -tetrazolium bromide; NOM = nomegestrol; PBS = phosphate-buffered saline; P-gp = P-glycoprotein; RT-PCR = reverse transcriptase polymerase chain reaction; TAM = tamoxifen; TopoIIα = topoisomerase IIα; VRP = verapamil.
Top67 and topoisomerase I recognised the same cleavage site on this substrate (Figure 4c).
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