Words similar to topoiiα

Example sentences for: topoiiα

How can you use “topoiiα” in a sentence? Here are some example sentences to help you improve your vocabulary:

ADR = adriamycin; DRO = droloxifen; GSTπ = glutathione S-transferase Pi; MDR = multidrug resistance; MG = megestrol; MRP = MDR-related protein; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H -tetrazolium bromide; NOM = nomegestrol; PBS = phosphate-buffered saline; P-gp = P-glycoprotein; RT-PCR = reverse transcriptase polymerase chain reaction; TAM = tamoxifen; TopoIIα = topoisomerase IIα; VRP = verapamil.
In brief, after three washes with phosphate-buffered saline (PBS), fixed cells in each smear were layered with the primary antibody against P-gp (1:20 dilution), GSTπ (1:25 dilution) and TopoIIα (1:50 dilution) and incubated overnight (a minimum of 18 h).
We also observed the expression of the non-P-gp MDR-related genes GSTπ, TopoIIα and MRP.
For MDR1, GSTπ and TopoIIα genes, after an initial denaturation at 94°C for 1.5 min, 30 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 94°C for 1.5 min, annealing at 54°C for 1 min, and extension at 72°C for 3 min, followed by a final step at 72°C for 10 min.
No significant change was found in TopoIIα expression level when measured on the second, third, fifth and tenth days after treatment in comparison with the control.