Example sentences for: topo

How can you use “topo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1TOPO (Invitrogen; acute O 3 library) or the Advantage PCR cloning vector (Clontech; all the other libraries) according to manufacturer's instructions.

  • Following the secondary PCR amplification, subtracted products from each tester cDNA population were cloned into the PCR IV vector of the TOPO TA cloning kit (Invitrogen, Carlsbad, CA) and used in the transformation of competent DH5α Escherichia coli cells.

  • Camptothecin (CPT) and its derivatives specifically target Topo I by binding noncovalently to these cleavable complexes, stabilizing them and interfering with DNA religation [ 11].

  • The nucleotide sequences of these genes contain specific DNA motifs that constitute high-affinity Topo I binding sites [ 31].

  • The amplified cDNA was cloned using the Invitrogen TOPO Cloning kit.


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