Words similar to topo

Example sentences for: topo

How can you use “topo” in a sentence? Here are some example sentences to help you improve your vocabulary:

We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.
Human DNA topoisomerase I (Topo I) is a 765-amino-acid nuclear enzyme [ 9] involved in topological changes of DNA structure [ 10].
Because of the potential pathogenic role of Topo I in SSc, we undertook this study to evaluate the utility of noncytotoxic concentrations of Topo I inhibitors for the treatment of SSc.
The eluted solution containing single strand, SSR-enriched fragments was cloned into the pCR4-TOPO vector, and the recombintants were transformed to TOP10 Chemically Competent E. coli following the instruction of TOPO TA Cloning kit (Invitrogen, San Diego, CA).
The nucleotide sequences of these genes contain specific DNA motifs that constitute high-affinity Topo I binding sites [ 31].