Words similar to topo

Example sentences for: topo

How can you use “topo” in a sentence? Here are some example sentences to help you improve your vocabulary:

PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).
We have focused on the effects of the leading Topo I inhibitor, CPT, on collagen production by dermal fibroblasts obtained from patients with SSc.
PCR products were subcloned into TOPO™ TA cloning vectors (Invitrogen) and subjected to cycle sequencing (Division of Molecular Transport, Department of Internal Medicine, University of Texas Southwestern Medical Center).
We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.
This study was undertaken to determine whether inhibition of its activity by noncytotoxic doses of CPT, a drug that selectively targets Topo I, could reverse the activated phenotype of fibroblasts from patients with SSc.