Example sentences for: topflash

How can you use “topflash” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First, co-transfection of the cDNA used to make our transgenic mice and the TOPFLASH Wnt pathway reporter plasmid into DAOY medulloblastoma cells resulted in over forty-fold induction of reporter activity.

  • In order to evaluate the transcriptional activity of FLAG epitope-tagged β-catenin in a medulloblastoma cell line, the S37F and wild-type constructs were introduced into DAOY cells along with the Wnt pathway TOPFLASH reporter.

  • This reporter plasmid contains three optimal TCF binding sites [ 28 ] . TOPFLASH luciferase activity was activated 41-fold by S37F mutant β-catenin and 3.8 fold by wild-type β-catenin in DAOY cells (Fig.

  • The TOPFLASH Wnt reporter plasmid was obtained from Dr. Kenneth Kinzler.


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