Words similar to ssc

Example sentences for: ssc

How can you use “ssc” in a sentence? Here are some example sentences to help you improve your vocabulary:

Isolation of total cellular RNA using a urea/LiCl protocol and gel electrophoresis was performed as described previously [ 65 ] . Gels were transferred onto Amersham Hybond N+ nylon membranes in 20 × SSC and prehybridized in 4 × SSC, 1 × Denhardt's solution, 0.5% SDS for 2 h at 68°C.
The embryos were processed as described previously [ 41] and hybridized to the probe overnight in 50% formamide, 5X SSC (pH 5.0), 50 μg/ml torula RNA, 50 μg/ml heparin at 70°C.
No major differences were observed at the three highest doses used (10 -8to 10 -6mol/l), but SSc fibroblasts appeared to be slightly more sensitive to the lowest dose of CPT (10 -9mol/l).
SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.
All probes were initially hybridized in a solution containing 55% formamide/2x SSC, 20% dextran sulfate, 1% Tween-20, incubated overnight at 37°C, and washed in 55% formamide/2x SSC at 42°C for 20 min, followed by four washes in 2x SSC at 37°C (2 min each) and 1-3 washes in 0.1x SSC at 60°C (1 min each).