Example sentences for: ssc

How can you use “ssc” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The 70-bp oligonucleotides were synthesized (Operon Technologies, CA), resuspended in 3 × SSC to a final concentration of 60 pmol/μl, and spotted onto poly-L-lysine-coated microscopic slides, as previously described [ 53].

  • Membranes were washed twice in 2x SSC, 1% SDS at 50°C for 20 min and once in 0.5x SSC, 1% SDS at 55°C for 15 min.

  • All probes were initially hybridized in a solution containing 55% formamide/2x SSC, 20% dextran sulfate, 1% Tween-20, incubated overnight at 37°C, and washed in 55% formamide/2x SSC at 42°C for 20 min, followed by four washes in 2x SSC at 37°C (2 min each) and 1-3 washes in 0.1x SSC at 60°C (1 min each).

  • After hybridization, microarray slides were washed by immersion into 2x SSC, 0.2% SDS for 5 min at 42°C, 0.2x SSC, 0.1% SDS for 1 min at room temperature, 0.2x SSC for 1 min at room temperature, and 0.05x SSC twice for 1 min at room temperature, dried by centrifugation, and immediately scanned.

  • After hybridization, sections were washed for 30 min in 2×SSC (room temperature), 1 hour in 2×SSC (65°C), 1 hr in 0.1×SSC (65°C), and equilibrated for 5 min in Buffer 1 (Tris-HCl 100 mM and NaCl 150 mM, pH 7.5).


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