Words similar to sscp

Example sentences for: sscp

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Existing procedures for detection of point mutations include SSCP (single strand conformational polymorphism) [ 11 12 ] , a variation of SSCP called DOVAM (Detection of Virtually All Mutations) [ 19 ] , Enhanced SSCP [ 28 ] , HA (Heteroduplex Analysis) [ 13 14 ] , PTT (Protein Truncation Test) [ 15 ] , DGGE (Denaturing Gradient Gel Electrophoresis) [ 16 17 18 ] , chemical cleavage of mismatch [ 20 ] , and RNase cleavage [ 21 ] . Each of these procedures has its advantages and limitations but the size of the gene and the cost of the procedures have not made them routine in most laboratories.
Furthermore, it should be noted that SSCP methodology does not detect all mutations of PCR products.
SSCP, single strand conformation polymorphism
[ 17 ] Briefly, the PCR products (4 μl) were mixed with SSCP sample buffer (6 μl, formamide with 0.05% bromophenol blue and xylene cyanol).
Single strand conformational polymorphism (SSCP) analysis was performed on all coding exons using primers flanking intronic sequences of the MGMT gene by standard methods.