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Example sentences for: srp

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Whereas Rpl11b-GFP was distributed relatively evenly in wild-type cells, it accumulated in the nucleus in a significant fraction of srp1-31 and net1 tab 2-1cells at 25°C and after 25°C ->37°C -> 25°C shift (Figure 6; [ 39 ] ). In addition, although mtr10 tab 1-1cells were relatively normal at 25°C, 30% of them accumulated nuclear Rpl11b after 25°C ->37°C -> 25°C shift (Figure 6).
Mtr10 assembles with and functions as a nuclear import receptor for the mRNA-binding protein Npl3 [ 36 37 ] . Interestingly, srp1-31 also inhibits nuclear import of multiple proteins including Npl3 [ 51 52 ] . Remarkably, reduction-of-function alleles of the karyopherin KAP104 were also recovered in a screen for mutations that suppressed cdc15-2 ts (A.
Third, both mtr10 tab 1-1and srp1-31 affect nuclear transport, suggesting that nuclear transport regulates mitotic exit.
The only phenotype known to be shared by srp1, mtr10, and kap104 mutants is accumulation of the 60S ribosomal protein Rpl11b in the nucleus [ 39 ] . SRP1, MTR10, and KAP104 may also collaborate to maintain the proper nucleocytoplasmic partitioning of α factor (or factors) that regulates mitotic exit.