Words similar to ssdna
Example sentences for: ssdna
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It has been suggested in the case of RecT that it may compete with SSB for binding single strand overhangs and thereby make them available for the annealing process [ 3 ] . Similar interactions between other SSAPs and SSB, that probably coats the ssDNA generated by nucleases, appear likely.
The molar ratio of reference template (ssDNA) to either intact template (dsDNA) or to cut template (dsDNA, considering only one of the two fragments) was about 10.
The similarity between these two systems is further extended by the observation that the RecT/E system can complement mutations in the λ exo/Redβ system [ 10 12 ] . In eukaryotes, RAD52 protein has been shown to exhibit properties similar to those of RecT and Redβ proteins: it binds ssDNA and promotes strand exchange via the pairing of complementary single strands [ 6 13 ] . In vitro studies on quaternary structures have shown that the single strand annealing proteins (SSAPs), RecT, Redβ, ERF and RAD52, form similar helical super-structures [ 14 15 16 17 ] . This has led to the proposal that RecT, Redβ, ERF and the eukaryotic RAD52 function in an analogous fashion, and even are "structural homologs" [ 14 ] .
The specific action of AlkB on ssDNA suggests that ssRNA could be the substrate for other members of this family.
On the basis of the preferential specificity of AlkB-dependent repair for ssDNA and for SN2 as opposed to SN1 alkylating agents, it has been proposed that its targets include modified bases such as N 1-methyladenine and N 3-methylcytosine [ 18].