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Example sentences for: ssdna
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Two samples with known quantities of templates were prepared: one sample contained the pBS (-) sequence template with a cut in the homologous region created by Pvu II digestion (cut template) and the intact ssDNA Phagescript DNA (reference template).
Both enzymes cleave only one DNA strand in a duplex and bacterial Xth cleaves ssDNA containing an abasic site [ 42 ] . The additional contacts huAPE1, compared to DNase 1 (Figure 6), makes 3' to the damage site and to the opposite strand lower its turnover rate and its potential to cleave normal DNA.
The similarity between these two systems is further extended by the observation that the RecT/E system can complement mutations in the λ exo/Redβ system [ 10 12 ] . In eukaryotes, RAD52 protein has been shown to exhibit properties similar to those of RecT and Redβ proteins: it binds ssDNA and promotes strand exchange via the pairing of complementary single strands [ 6 13 ] . In vitro studies on quaternary structures have shown that the single strand annealing proteins (SSAPs), RecT, Redβ, ERF and RAD52, form similar helical super-structures [ 14 15 16 17 ] . This has led to the proposal that RecT, Redβ, ERF and the eukaryotic RAD52 function in an analogous fashion, and even are "structural homologs" [ 14 ] .
These proteins are known to cooperate with SbcD, nuclease of the calcineurin-like phosphoesterase superfamily and to degrade dsDNA in the 3' → 5' direction generating ssDNA [ 35 36 ] . It seems likely that RecT/Redβ proteins, at least in certain cases, function in conjunction with the SbcCD-pathway, by utilizing the single-stranded regions generated by the SbcCD nuclease.
In at least one case, each of them was found adjacent to the gene for the single-strand-binding protein (SSB), an OB-fold protein that binds ssDNA (Figs.
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