Example sentences for: secondary

How can you use “secondary” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 025% Triton, 1x PBS); incubated for 2 hours at 4°C with Cy3 conjugated anti-rabbit secondary antibody (Jackson ImmunoResearch) and Alexa-488 conjugated anti-mouse secondary antibody (Molecular Probes Inc.) diluted 1:300 in blocking buffer; washed 3 × for 15 minutes in wash buffer and mounted with Immumount (Shandon).

  • The antibodies were visualized using donkey anti rabbit and donkey anti-sheep secondary antibodies (Jackson; 1:50), labeled with FITC and RITC, respectively.

  • The monoclonal mAb414 [ 68 ] and anti-Pom152 monoclonal mAb118C3 [ 69 ] were detected with FITC Goat-anti-mouse secondary.

  • Previous studies have shown that adoptively transferred tumor antigen-specific CD4 +T cells can be tolerized in the secondary lymphoid organs [ 9 34 ] , including the RIP-Tag2 model [ 30 ] . Radiation may kill or disable regulatory T cells [ 35 36 ] , or other bone marrow-derived tolerizing cells and prevent the induction of tolerance in adoptively transferred tumor-specific T cells [ 9 ] . Additionally, the high but sub-lethal dose of radiation used leads to significant lymphopenia [ 37 ] . The sudden reduction in lymphocyte number may create the spatial allowance in spleen and lymph nodes necessary for the homing, activation and expansion of he adoptively-transferred T cells.

  • However, some of these features may be secondary to cytoreductive therapy and other components of BMT.


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