Words similar to second-strand

Example sentences for: second-strand

How can you use “second-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).
We also observed this pattern, albeit not as robust, at the 3' end of the pre-insertion locus, suggesting that L1 endonuclease may also be responsible for the second-strand cleavage of host DNA during L1 insertion.
Before the second-strand synthesis, 200-500 ng oligo(dT) 24 -T7 primer was added and denatured at 65°C for 10 min.
Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.
Second-strand cDNA was synthesized in a final volume of 150 μl, in a mixture containing 30 μl of 5X second-strand buffer, 3 μl of 10 mM dNTP mix, 4 μl of E. coli DNA polymerase I (10 U/μl) and 1 μl of RNase H (2 U/μl) for 2 hours at 16°C.