Words similar to second-strand

Example sentences for: second-strand

How can you use “second-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.
Before the second-strand synthesis, 200-500 ng oligo(dT) 24 -T7 primer was added and denatured at 65°C for 10 min.
Cold second-strand premix (64.
The second-round second-strand synthesis, clean-up and IVT were carried out as for the first round, except that no ligase was added to the second-strand premix.
We also observed this pattern, albeit not as robust, at the 3' end of the pre-insertion locus, suggesting that L1 endonuclease may also be responsible for the second-strand cleavage of host DNA during L1 insertion.