Words similar to second-strand

Example sentences for: second-strand

How can you use “second-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

The second-round second-strand synthesis, clean-up and IVT were carried out as for the first round, except that no ligase was added to the second-strand premix.
Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.
5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).
After first- and second-strand synthesis, the full-length cDNAs were selected, size fractionated and cloned into pBluescript.
Cold second-strand premix (64.