Words similar to second-strand

Example sentences for: second-strand

How can you use “second-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

This second round of cDNA synthesis was similar to the first round except that random hexamers were used in priming of first-strand synthesis, with T7-(dT) 24 oligomer priming the second-strand.
Second-strand synthesis was primed by the partially digested RNA that resulted from RNase H treatment of the first-strand synthesis reaction.
Cold second-strand premix (64.
Before the second-strand synthesis, 200-500 ng oligo(dT) 24 -T7 primer was added and denatured at 65°C for 10 min.
Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.