Words similar to second-strand

Example sentences for: second-strand

How can you use “second-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

Second-strand synthesis was primed by the partially digested RNA that resulted from RNase H treatment of the first-strand synthesis reaction.
Second-strand cDNA was synthesized in a final volume of 150 μl, in a mixture containing 30 μl of 5X second-strand buffer, 3 μl of 10 mM dNTP mix, 4 μl of E. coli DNA polymerase I (10 U/μl) and 1 μl of RNase H (2 U/μl) for 2 hours at 16°C.
5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).
Cold second-strand premix (64.
We also observed this pattern, albeit not as robust, at the 3' end of the pre-insertion locus, suggesting that L1 endonuclease may also be responsible for the second-strand cleavage of host DNA during L1 insertion.