Words similar to second-stage

Example sentences for: second-stage

How can you use “second-stage” in a sentence? Here are some example sentences to help you improve your vocabulary:

In the second-stage PCR, aliquots of the purified products from the first amplification (0.
Embryos develop in a proteinaceous matrix extruded by the adult female, and hatch as second-stage larvae (L2) that move through the soil and invade the plant root.
The generation of expressed sequence tags (ESTs) by single-pass random sequencing of cDNA libraries is a powerful tool for rapid gene transcript identification in metazoans [ 14 15 16 17 ] including parasitic nematodes of humans and animals [ 18 19 20 21 22 23 ] . High-throughput projects on two dozen nematode species have now brought the total number of publicly available roundworm ESTs to nearly 400,000, with half the sequences coming from parasites [ 24 25 26 27 ] . As a part of these efforts, EST sequencing from plant parasitic nematodes is in progress [ 28 ] and pilot EST datasets from the root-knot nematode M. incognita and the cyst nematodes Globodera rostochiensis and G. pallida second-stage larvae have recently been analyzed [ 29 30 ] .
The Meloidogyne L2 larvae, the infective stage where the worm is away from the host plant (also referred to as second-stage juvenile in the literature), is more accessible than the rest of the life cycle, and is an interesting stage biologically with the worm completing multiple steps required for survival.