Example sentences for: seconds

How can you use “seconds” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following the incubation period, samples were centrifuged at 9 000 × g for 30 seconds and the supernatants collected.

  • To measure inactivation, cells were held at -80 mV, then stepped to a series of depolarizing (pre-pulse) voltages, from -70 mV to +70 mV in 10 mV increments, for 2.5 seconds to promote inactivation, then stepped to -50 mV for 100 ms to close non-inactivated channels, then stepped for 200 ms to the test potential of +20 mV to measure Ca 2+currents, then stepped to the -80 mV holding potential for 5 seconds to permit recovery from inactivation.

  • All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.

  • The entire process took about 15 seconds.

  • This report from the C-130H was recorded on ATC audio about 1 minute and 37 seconds after the impact time of United 93 as established by NTSB and Commission analysis of FDR, CVR, radar, and impact data sets-more than a minute before the earliest impact time originally posited by the authors of the seismic data report.


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