Words similar to rnasin
Example sentences for: rnasin
How can you use “rnasin” in a sentence? Here are some example sentences to help you improve your vocabulary:
82 mg/ml), 0.5 μl RNasin (Promega), 1 μl Superscript II (Invitrogen)).
For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).
Adult mouse testes were homogenized in a buffer containing 20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3% NP-40, 40 U/ml of Rnasin ribonuclase inhibitor (Promega, Madison, WI), and a mixture of 10 protease inhibitors provided in the Protease Inhibitors Set (Roche Molecular Biochemicals, Indianapolis, IN).
Forty units of a ribonuclease inhibitor (RNAsin) (Promega, Madison, WI) were added.
The RNA/primer mixture was denatured at 70°C for 3 min and cooled on ice for 2 min, followed by adding: 4 μl of 5X first strand buffer (Invitrogen™), 2 μl 0.1 M DTT, 1 μl RNasin ®(40 U/μl, Promega™), 2 μl 10 mM dNTP, and 2 μl Superscript™ II (200 U/μl, Invitrogen™), and incubated at 42°C for 1.5 hours.
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