Words similar to rnasin
Example sentences for: rnasin
How can you use “rnasin” in a sentence? Here are some example sentences to help you improve your vocabulary:
82 mg/ml), 0.5 μl RNasin (Promega), 1 μl Superscript II (Invitrogen)).
Adult mouse testes were homogenized in a buffer containing 20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3% NP-40, 40 U/ml of Rnasin ribonuclase inhibitor (Promega, Madison, WI), and a mixture of 10 protease inhibitors provided in the Protease Inhibitors Set (Roche Molecular Biochemicals, Indianapolis, IN).
RNasin Ribonuclease Inhibitor (Promega) was used in both 5'-RACE and RT-PCR reactions.
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
The reaction was performed in endogenous polymerase buffer (50 mM Tris-HCl pH 8.0, 100 mM NaCl, 0.1% NP-40, 10 mM MgCl 2 2.5% 2-mercaptoethanol) with 1 μg oligonucleotide, 100 ng purified radiolabeled RNA template, and 4 U RNasin at 37°C for 65 min.
Loading...