Example sentences for: rnasin

How can you use “rnasin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The RNA/primer mixture was denatured at 70°C for 3 min and cooled on ice for 2 min, followed by adding: 4 μl of 5X first strand buffer (Invitrogen™), 2 μl 0.1 M DTT, 1 μl RNasin ®(40 U/μl, Promega™), 2 μl 10 mM dNTP, and 2 μl Superscript™ II (200 U/μl, Invitrogen™), and incubated at 42°C for 1.5 hours.

  • Adult mouse testes were homogenized in a buffer containing 20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3% NP-40, 40 U/ml of Rnasin ribonuclase inhibitor (Promega, Madison, WI), and a mixture of 10 protease inhibitors provided in the Protease Inhibitors Set (Roche Molecular Biochemicals, Indianapolis, IN).

  • Forty units of a ribonuclease inhibitor (RNAsin) (Promega, Madison, WI) were added.

  • One μl of RNasin was added to the suspension, and the RNA concentration was determined by spectrophotometry.

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.


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