Example sentences for: rnasin

How can you use “rnasin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2 , 0.001% gelatin, 1 mM of each dNTP, 5 mM dithiothreitol, 2.9 U RNasin, 20 pmoles of primer SP-BR (5'-GAATCACAGCTTGGACCCGC-3'), and 16 U MMLV reverse transcriptase.

  • 82 mg/ml), 0.5 μl RNasin (Promega), 1 μl Superscript II (Invitrogen)).

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.

  • For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).

  • 22 units of AMV RT enzyme, and 20 units of RNAsin per reaction.


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