Words similar to rnasin
Example sentences for: rnasin
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82 mg/ml), 0.5 μl RNasin (Promega), 1 μl Superscript II (Invitrogen)).
The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
One μl of RNasin was added to the suspension, and the RNA concentration was determined by spectrophotometry.
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
2 , 0.001% gelatin, 1 mM of each dNTP, 5 mM dithiothreitol, 2.9 U RNasin, 20 pmoles of primer SP-BR (5'-GAATCACAGCTTGGACCCGC-3'), and 16 U MMLV reverse transcriptase.