Words similar to rnasin
Example sentences for: rnasin
How can you use “rnasin” in a sentence? Here are some example sentences to help you improve your vocabulary:
RNA for cDNA production was performed using Promega's (Madison, WI) random hexamers, RNAsin, RT buffer, and RT enzyme.
The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
To this, 4 μl of a 5×AMV RT reaction buffer, 2 μl of 25 mM MgCl, 2 μl of 10 mM dNTP, 0.5 μl of Rnasin (1 U/μl) (Promega), 1 μl of oligo(dT) 15 (0.
82 mg/ml), 0.5 μl RNasin (Promega), 1 μl Superscript II (Invitrogen)).
Adult mouse testes were homogenized in a buffer containing 20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3% NP-40, 40 U/ml of Rnasin ribonuclase inhibitor (Promega, Madison, WI), and a mixture of 10 protease inhibitors provided in the Protease Inhibitors Set (Roche Molecular Biochemicals, Indianapolis, IN).
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