Example sentences for: rnase

How can you use “rnase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In cultured cells RNase protection assay detected EP2 expression in renal medullary interstitial cells (MICs), while no signal was detected in cultured cortical collecting ducts or glomerular mesangial cells (Fig.

  • However, there are few studies where the majority of the gene sequences spotted on the microarrays were verified [ 5 ] . Kuo et al (2002) compared the data from two high-throughput DNA microarray technologies, cDNA microarray (Stanford type) and oligonucleotide microarray (from Affymetrix) and found very little correlation between these two platforms [ 6 ] . Unfortunately, many investigators are reporting microarray data without confirming their results by other traditional gene expression techniques such as PCR, Northern blot analysis and RNase protection assay.

  • Second strand cDNA is synthesized by degrading the poly(A) +RNA strand with RNase H, followed by second strand synthesis with E. coli DNA polymerase I. Amplified antisense RNA (aRNA) is obtained from in vitro transcription of the double-stranded cDNA (ds cDNA) template using T7 RNA polymerase.

  • RNase protection assays were performed using RNA from three separate human cell lines, MRC5, MCF7, and BT20.

  • Members of the ribonuclease III superfamily of endoribonucleases [ 1 ] are the primary agents of dsRNA cleavage [ 2 ] . RNase III orthologues are conserved in eukaryotes and in bacteria, with Escherichia coli RNase III [ 3 ] as the best characterized member.


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