Words similar to rnase
Example sentences for: rnase
How can you use “rnase” in a sentence? Here are some example sentences to help you improve your vocabulary:
In cultured cells RNase protection assay detected EP2 expression in renal medullary interstitial cells (MICs), while no signal was detected in cultured cortical collecting ducts or glomerular mesangial cells (Fig.
However, there are few studies where the majority of the gene sequences spotted on the microarrays were verified [ 5 ] . Kuo et al (2002) compared the data from two high-throughput DNA microarray technologies, cDNA microarray (Stanford type) and oligonucleotide microarray (from Affymetrix) and found very little correlation between these two platforms [ 6 ] . Unfortunately, many investigators are reporting microarray data without confirming their results by other traditional gene expression techniques such as PCR, Northern blot analysis and RNase protection assay.
Second strand cDNA is synthesized by degrading the poly(A) +RNA strand with RNase H, followed by second strand synthesis with E. coli DNA polymerase I. Amplified antisense RNA (aRNA) is obtained from in vitro transcription of the double-stranded cDNA (ds cDNA) template using T7 RNA polymerase.
RNase protection assays were performed using RNA from three separate human cell lines, MRC5, MCF7, and BT20.
Members of the ribonuclease III superfamily of endoribonucleases [ 1 ] are the primary agents of dsRNA cleavage [ 2 ] . RNase III orthologues are conserved in eukaryotes and in bacteria, with Escherichia coli RNase III [ 3 ] as the best characterized member.