Example sentences for: rnase-free

How can you use “rnase-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).

  • Unincorporated nucleotides were removed by size-exclusion chromatography on RNase-free Sephadex G-50 (Sigma) spin columns.

  • RNA from S2 cells and mbn-2 cells was prepared using Qiagen RNA miniprep columns and was treated with RQ1 RNase-Free DNase (Promega).

  • RNA was first mixed with either 8 μg of random primer for aRNA or 5 μg of oligo(dT) primer for poly(A) +RNA in 16 μl of RNase-free water.

  • The RNA sample was added to a sterile RNase-free micro-centrifuge tube, and nuclease-free water was added to 9.5 μl.


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