Example sentences for: rnase-free

How can you use “rnase-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For first strand cDNA synthesis, 3 μg (unless otherwise specified) tumor total RNA, Universal Human Reference total RNA (Stratagene ®), or 150 ng poly(A) +RNA was mixed with 1 μg Eberwine primer (Operon ®) in RNase-free water to a total volume of 9 μl.

  • The pellet was washed with 75% ethanol and resuspended in RNase-free water.

  • Clean-up was performed with DNA clean and concentrator-5 (Zymo Research), eluting twice with 8 μl RNase-free water.

  • After in vitro transcription, 20 U of RNase-free DNase I was added to digest the DNA templates, and the labeled riboprobes were precipitated with ethanol and then resuspended in 100 μl of DEPC-treated water.

  • The pellet was washed in 500 μl 75% ethanol, centrifuged at 16,000 g for 5 min, air dried and resuspended in 16 μl RNase-free water.


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