Words similar to rnase-free
Example sentences for: rnase-free
How can you use “rnase-free” in a sentence? Here are some example sentences to help you improve your vocabulary:
After in vitro transcription, 20 U of RNase-free DNase I was added to digest the DNA templates, and the labeled riboprobes were precipitated with ethanol and then resuspended in 100 μl of DEPC-treated water.
RNA from S2 cells and mbn-2 cells was prepared using Qiagen RNA miniprep columns and was treated with RQ1 RNase-Free DNase (Promega).
Total RNA from larval tissues was extracted using the Mini RNA Isolation Kit (Zymo Research, Orange, CA) and was eluted with RNase-free water.
5 μl prepared on ice: 45 μl RNase-free water, 15 μl 5× second-strand buffer (Invitrogen), 1.5 μl dNTPs (10 mM each), 0.5 μl E. coli ligase (10 U/μl), 2 μl E. coli polymerase (10 U/μl), 0.5 μl E. coli RNaseH (2 U/μl)).
The pellet was washed in 500 μl 75% ethanol, centrifuged at 16,000 g for 5 min, air dried and resuspended in 16 μl RNase-free water.
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