Words similar to rnaseh
Example sentences for: rnaseh
How can you use “rnaseh” in a sentence? Here are some example sentences to help you improve your vocabulary:
Second, to limit the possibility that the failure to detect RNAseH activity was due to insufficient number of viral core particles, an RNAseH assay was performed with a 10-fold increase in cores.
With the mixture remaining on ice, 4 μl of 5X first-strand buffer, 2 μl 0.1 M DTT, 1 μl of 10 mM dNTP mix and 1 μl Superscript™ II RNaseH -reverse transcriptase (200 U/μl) was added to make a final volume of 20 μl, and the mixture incubated for one hour in a 42°C water bath.
RNAseH, ribonuclease H
The RNAseH substrate itself (DRF+ RNA annealed to D2507-) also supported DNA synthesis in the trans assay (lane 4), as did the combination of DRF+ RNA and the non-complementary oligonucleotide D2526+ (lane 5).
DHBV RNAseH cleavage was not detected within the cores under these conditions (data not shown).