Words similar to rnaseh

Example sentences for: rnaseh

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Viral cores prepared from LMH cells transfected with DHBV, DHBV-D715V, and DHBV-YMHA were employed with DRF+ RNA in the oligonucleotide-directed RNAseH assay in the first attempt to detect DHBV RNAseH activity on exogenous substrates.
During synthesis of the minus-strand DNA the pregenomic RNA is degraded by the ribonuclease H (RNAseH) activity of P. However, the RNAseH activity does not digest the extreme 5' end of the template RNA, and this ∼ 18 nucleotide capped RNA fragment is then transferred to direct repeat 2 (DR2), where it acts as a primer for synthesis of plus-strand DNA [ 11, 12].
The aim of this study was to determine if the DHBV RNAseH activity was similarly restricted to the endogenous heteroduplex substrate generated during reverse transcription.
RNAseH assays were also carried out after removal of the endogenous nucleic acid, and multiple RNA sequences and DNA oligonucleotide combinations were employed.
However, this cannot entirely account for the substrate commitment because removal of endogenous nucleic acid with micrococcal nuclease only partially relieves the template commitment of the DNA polymerase activity and it did not relieve the apparent restriction on the RNAseH activity.