Words similar to pst

Example sentences for: pst

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Smaller pieces of the sequence upstream of the Pst I site are also sufficient to induce ALM migration defects (Fig.
To enrich for clones with increased survival in human macrophages, the library was passed through THP-1 macrophages as shown schematically in Figure 1. THP-1 is a human monocyte-derived macrophage cell line [ 21 ] and wild-type M. smegmatis bacteria are rapidly killed by THP-1 macrophages [ 22 ] . After three rounds of enrichment, the resulting clones were analyzed individually by Southern blot to evaluate the number of different clones present as previously described [ 20 ] . Out of the 3000 colonies recovered, genomic DNAs from the bacteria of 90 randomly chosen colonies were digested with Pst I and hybridized with the pYUB178 vector.
The 495 bp region from the 5' end of the mec-3 clone to the Pst I site appeared to most strongly affect ALM migrations (compare pJC3 and pJC4 to pPD118.
Microdissection of nephron segments was performed in the kidney of New Zealand White rabbit as previously described [ 44 ] . The following segments were dissected: glomeruli, proximal convoluted tubules (PCT), proximal straight tubules (PST), thin limbs of Henle's loop (tDL/tAL), thick ascending limb (TAL), distal convoluted tubules (DCT), cortical collecting ducts (CCD) and medullary collecting duct (MCD) cells.
DNA was isolated from PdL lines and restriction digested with Xba I, Hind III, Pst I and Taq I in separate reactions.