Example sentences for: pst

How can you use “pst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PST, Friday, Oct. 29.)

  • EST/PST).

  • The rat VEGF probe is a 0.9 kb cDNA Pst I/Sma I insert cloned into a pBluescript II KS+ vector [ 33 ] . Prehybridization and hybridization were performed in 50% formamide, 5×SSC (20×SSC is 0.3 M sodium chloride, 0.3 < sodium citrate), 10× Denhardt's solution (50× Denhardt's solution is 2% Ficoll, 2% polyvinyl pyrrolidone, 2% Bovine Serum Albumin Factor V), 50 mM sodium phophate (pH 7.0), 1% sodium dodecyl sulfate (SDS), and 250 μg/ml salmon sperm DNA at 42°C.

  • Probe CHRNA7 - Pst I was designed by identifying an approximately 100 bp RFLP between the CHRNA7 -related sequence within accessions AC010799 (RP11-40J8) and AC111169 (RP11-20D7), using the web-based restriction mapping program Webcutter 2.0 [ 36 ] . Once a polymorphism distinguishing the two copies of CHRNA7 in Homo sapiens had been identified, probe CHRNA7 - Pst I was PCR amplified from BAC RP11-40J8 DNA using primers CHRNA7 .probe2.

  • To determine whether having a promoter present increased the effectiveness of this sequence, we made two plasmids shown in Figure 6. Plasmid pJC11 had the region upstream of the Pst I site fused to the region of the mec-3 promoter, whereas pJC10 had just the mec-3 region upstream of the Pst I site.


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