Words similar to psti

Example sentences for: psti

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The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.
DNA encoding the full-length human cPLA 2 was cloned into the vector pEGFP-C3 (Clontech) to create pEGFP-cPLA 2 , as previously described [ 15 ] . The XbaI/PstI fragment from a cPLA 2 α clone containing S505A or S727A mutations [ 13 ] was inserted into an XbaI/PstI site in pEGFP-cPLA 2 to generate pEGFP-cPLA 2 S505A and pECFP-cPLA 2 S727A.
To further narrow down the sequences that are responsible for expression in myocytes exon 1b was directly linked to the reporter using a PstI site 10 nucleotides downstream from the transcription start site (see clone CH211-251J8).
The resulting plasmid was digested with SacI and PstI to remove the 5' MCS, which was replaced by a PacI linker.