Words similar to psti

Example sentences for: psti

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DNA encoding the full-length human cPLA 2 was cloned into the vector pEGFP-C3 (Clontech) to create pEGFP-cPLA 2 , as previously described [ 15 ] . The XbaI/PstI fragment from a cPLA 2 α clone containing S505A or S727A mutations [ 13 ] was inserted into an XbaI/PstI site in pEGFP-cPLA 2 to generate pEGFP-cPLA 2 S505A and pECFP-cPLA 2 S727A.
The resulting plasmid was digested with SacI and PstI to remove the 5' MCS, which was replaced by a PacI linker.
The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.
To further narrow down the sequences that are responsible for expression in myocytes exon 1b was directly linked to the reporter using a PstI site 10 nucleotides downstream from the transcription start site (see clone CH211-251J8).