Example sentences for: proteasome

How can you use “proteasome” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 20 μM lactacystin (Sigma, St. Louis, MO) was added to the medium 18 h after transfection and cells were incubated with the proteasome inhibitor for 4 h prior to harvest.

  • As an outcome of this requirement, longer polyubiquitin tags would be preferential substrates for degradation by the proteasome.

  • In addition, the use of proteasome inhibitors (Figures 7and 9) suggest that sα i2 is degraded by a proteasome pathway.

  • This system employs a cascade of enzymatic reactions that covalently attach a ubiquitin chain to substrate proteins, thereby targeting them to the proteasome [ 19 ] . The ubiquitin transfer reaction involves three enzymes: E1, which mediates the ATP-dependent activation of ubiquitin, and E2, or ubiquitin conjugating enzyme (UBC), which, together with an E3 ubiquitin ligase, transfers ubiquitin to the target protein.

  • When proteasome inhibitors were applied, the inhibitors LLnL (50 mM in DMSO) or lactacystin (10 mM in H 2 O) were added directly to the cell medium at 1:1000 dilutions.


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