Words similar to proteasome
Example sentences for: proteasome
How can you use “proteasome” in a sentence? Here are some example sentences to help you improve your vocabulary:
20 μM lactacystin (Sigma, St. Louis, MO) was added to the medium 18 h after transfection and cells were incubated with the proteasome inhibitor for 4 h prior to harvest.
As an outcome of this requirement, longer polyubiquitin tags would be preferential substrates for degradation by the proteasome.
In addition, the use of proteasome inhibitors (Figures 7and 9) suggest that sα i2 is degraded by a proteasome pathway.
This system employs a cascade of enzymatic reactions that covalently attach a ubiquitin chain to substrate proteins, thereby targeting them to the proteasome [ 19 ] . The ubiquitin transfer reaction involves three enzymes: E1, which mediates the ATP-dependent activation of ubiquitin, and E2, or ubiquitin conjugating enzyme (UBC), which, together with an E3 ubiquitin ligase, transfers ubiquitin to the target protein.
When proteasome inhibitors were applied, the inhibitors LLnL (50 mM in DMSO) or lactacystin (10 mM in H 2 O) were added directly to the cell medium at 1:1000 dilutions.
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