Example sentences for: polytron

How can you use “polytron” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Tissues were homogenized with a Polytron sonicating homogenizer (PT10/35, Brinkman Instrument Inc, Westbury, NY), and RNA was extracted with TRIzol (Gibco BRL, Gaithersburg, MD).

  • Supernatants were discarded and the pellets were resuspended in 10% sucrose, 50 mM Tris pH 7.5, 5 mM MgCl, 1 mM EDTA solution using three 10-second pulses with a Polytron (Brinkman; Westbury, USA) at a power setting of 12.

  • At a later date, frozen livers, colon or tumor were thawed and homogenized individually at 4°C in 280 mM mannitol with 10 mM HEPES buffer with 0.01% BHT using a Polytron homogenizer.

  • Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.

  • The cortex of the kidneys was minced with a tissue press and homogenized using a Polytron homogenizer at moderate speed for 15 s ×2.


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