Words similar to phycoerythrin
Example sentences for: phycoerythrin
How can you use “phycoerythrin” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cRNA was hybridized for 16 hours to an array, followed by binding with a streptavidin-conjugated fluorescent marker, and then incubated with a polyclonal anti-streptavidin antibody coupled to phycoerythrin as an amplification step.
The probe arrays were then stained by adding 80 μl staining solution (100 mM MES, 1 M NaCl, 0.05% Tween-20 with 2 mg/ml acetylated BSA (Sigma, St Louis, MO) and 10 μg/ml streptavidin R-phycoerythrin (Molecular Probes, Portland, OR) for 30 min at 25°C.
2-, 3- or 4-color flow cytometric analysis was performed on all samples following a standard red cell lysis method using mouse monoclonal antibodies directed against CD45 (clone 2D1, peridin-chlorophyll-a-protein [PerCP]-conjugated,) as well as various combinations of CD26 (fluorescien isothiocyanate [FITC]-conjugated), CD3 (SK7, FITC or phycoerythrin [PE]), CD4 (SK3, PE), CD7 (4H9, FITC), CD8 (SK1, FITC or PE), CD19 (SJ25C1, FITC) and CD26 (L272, FITC).
To the cell pellet, 3 ul of phycoerythrin (PE) conjugated mouse anti-human IFN-γ or TNF-α monoclonal Ab (Pharmingen Inc.) was added and incubated 30 min at 4°.
Microarrays were washed and sequentially incubated with streptavidin phycoerythrin (Molecular Probes, Eugene, OR), biotinylated anti-streptavidin antibody (Vector Laboratories, Inc., Burlingame, CA), and streptavidin phycoerythrin on the Fluidic Station (Affymetrix, Santa Clara, CA).
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