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Example sentences for: pgf
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Eicosanoids were quantified by measuring the ratio of the intensity of ions m/z 569/573 for PGF 2α , m/z 614/618 for TXB 2 and 6-keto-PGF 1α , and m/z 524/528 for PGE 2 . An analytical blank for each of these products was determined by measuring the amount of nondeuterated material, detected after extracting and analyzing 2 ml of saline to which the deuterium-labeled internal standards had been added.
However, the microvasculature of the CL is composed of a number of different luteal endothelial cell types; the most common of which does not express mRNA encoding the PGF
COX-2 activity was determined by measuring PGE 2 , 6-keto-PGF 1α (stable PGI 2 metabolite), TXB 2 (stable TXA 2 metabolite), and PGF 2α production in stimulated fibroblasts.
After evaporation of reagents, the residue was subjected to TLC plates, using the solvent system chloroform/ethanol (93:7, vol/vol) for PGF 2α and TXB 2 , and ethyl acetate/methanol (93:2, vol/vol) for 6-keto-PGF 1α and PGE 2 . Then PGF 2α was converted to trimethylsilyl ether derivative by treatment with N,O-bis (trimethylsilyl) trifluoroacetamide and dimethylformamide.
2α receptor FP [ 35 44 ] . This particular endothelial cell type is unresponsive to PGF 2α treatment [ 35 ] in that PGF 2α treatment does not increase the secretion of monocyte chemoattractant protein-1 [ 35 ] or stimulate well-recognized PGF 2α -responsive intracellular signaling pathways [ 35 36 ] . Lehmann, et al . [ 45 ] recently characterized a predominant cytokeratin-negative endothelial cell type in the bovine CL in addition to a "rare" cytokeratin-positive endothelial cell type.
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