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Example sentences for: pgf

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Prostanoids were measured by modified stable isotope dilution assays that used gas chromatography-negative ion-chemical ionization mass spectrometry as previously described [ 23 ] . Briefly, deuterium-labeled internal standards of PGE 2 , PGF 2α , TXB 2 , and 6-keto-PGF 1α were added to the supernatants with isopropyl alcohol.
Consequently, there is some question as to whether PGF 2α has any direct effect on CLENDO cell function, which directed our attention toward other known luteolytic factors ( i.e. , cytokines).
The methoxime derivative of TXB 2 , PGE 2 and 6-keto-PGF 1α was made by treatment with 2% methoxamine hydrochloride in pyridine at 70°C for 60 min, followed by evaporation of the pyridine, addition of water, and extraction with ethyl acetate.
Treatment of animals with PGF 2α or its analogs results in endothelial cell death in vivo [ 43 ] , suggesting that PGF 2α may have a direct effect on CLENDO cells.
After evaporation of reagents, the residue was subjected to TLC plates, using the solvent system chloroform/ethanol (93:7, vol/vol) for PGF 2α and TXB 2 , and ethyl acetate/methanol (93:2, vol/vol) for 6-keto-PGF 1α and PGE 2 . Then PGF 2α was converted to trimethylsilyl ether derivative by treatment with N,O-bis (trimethylsilyl) trifluoroacetamide and dimethylformamide.